Voltage-dependent gap junction channels are formed by connexin32, the major gap junction protein of rat liver.
Voltage-dependent gap junction channels are formed by connexin32, the major gap junction protein of rat liver.
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DOI：
10.1016/s0006-3495(91)82305-0
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发表时间：
1991-04
影响因子：
3.4
通讯作者：
Alonso P. Moreno;A. C. C. D. Carvalho-A.-C.-C.-D.-Carvalho-2259865318;V. Verselis;B. Eghbali;David C. Spray;Z. Gatmaitan;R. L. White;A. C. C. D. Carvalho-A.-C.-C.-D.-Carvalho-2259865318
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作者：
Alonso P. Moreno;A. C. C. D. Carvalho-A.-C.-C.-D.-Carvalho-2259865318;V. Verselis;B. Eghbali;David C. Spray;Z. Gatmaitan;R. L. White;A. C. C. D. Carvalho-A.-C.-C.-D.-Carvalho-2259865318
我们在此报告了在成对肝细胞中进行的实验，这些实验证明了连接电导的显著电压敏感性，因此与本实验室报告的早期发现相矛盾（Spray，D.C.，R.D.金兹伯格，EA，E. A. Morales，Z. Gatmaitan和I.M.阿里亚斯，1986，细胞生物学杂志101：135-144; Spray C.D. R.L.白色，AC Campos de Carvalho和M.V.L.班尼特。1984. Biophys. J. 45：219-230）和其他人（Dahl，G.，T. Moller，D.保罗河Voellmy和R.沃纳1987.科学（Wash）DC] 236：1290-1293; Riverdin，E.C.，和R.温加特1988. Am. 254：C226-C234）。在外源系统中的表达，其中掺入分离的间隙连接膜的片段的脂质双层（Young，J.D. E、Z. Cohn和N.B.吉鲁拉1987. Cell. 48：733-743.）和用连接蛋白32 cDNA转染的非通讯细胞（Eghbali，B.，J.A.凯斯勒和特区喷雾1990. Proc. Natl. Acad. Sci. USA. 87：1328-1331），支持这些发现，并表明电压依赖性通道由连接蛋白32（大鼠肝脏的主要间隙连接蛋白）组成（Paul，D. 1986. 103：123-134）。
We report here experiments undertaken in pairs of hepatocytes that demonstrate a marked voltage sensivity of junctional conductance and, thus, contradict earlier findings reported by this laboratory (Spray, D.C., R.D.ginzberg, E.A., E. A. Morales, Z. Gatmaitan and I.M. Arias, 1986, J. Cell Biol. 101:135–144; Spray C.D. R.L. White, A.C. Campos de Carvalho, and M.V.L. Bennett. 1984. Biophys. J. 45:219–230) and by others (Dahl, G., T. Moller, D. Paul, R. Voellmy, and R. Werner. 1987. Science [Wash. DC] 236:1290–1293; Riverdin, E.C., and R. Weingart. 1988. Am. J. Physiol. 254:C226-C234). Expression in exogenous systems, lipid bilayers in which fragments of isolated gap junction membranes were incorporated (Young, J.D.-E., Z. Cohn, and N.B. Gilula. 1987. Cell. 48:733–743.) and noncommunicating cells transfected with connexin32 cDNA (Eghbali, B., J.A. Kessler, and D.C. Spray. 1990. Proc. Natl. Acad. Sci. USA. 87:1328–1331), support these findings and indicate that the voltage-dependent channel is composed of connexin32, the major gap junction protein of rat liver (Paul, D. 1986. J. Cell Biol. 103:123–134).