Morphine-induced conditioned place preference in mice: Metabolomic profiling of brain tissue to find "molecular switch" of drug abuse by gas chromatography/mass spectrometry
Morphine-induced conditioned place preference in mice: Metabolomic profiling of brain tissue to find "molecular switch" of drug abuse by gas chromatography/mass spectrometry
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DOI：
10.1016/j.aca.2011.09.033
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发表时间：
2012-01-13
影响因子：
6.2
通讯作者：
Mao, Yu
中科院分区：
文献类型：
--
作者：
Meng, Jiaoran;Zhang, Xiaodong;Mao, Yu
条件位置偏好（CPP）是一个被广泛应用的情境依赖学习模型。本研究采用GC-MS联用技术对小鼠脑内代谢产物进行了研究，并将其用于吗啡奖赏效应相关的情境依赖性学习机制的研究。从脑组织中提取甲硫氨酸并衍生化，然后通过气相色谱/质谱（GC-MS）进行分析。总共有69个峰被鉴定为已知化合物。通过Wilcoxon秩和检验，p值
Conditioned place preference (CPP) is a widely used model to explore the mechanism of context-dependent learning. In this work, we developed a GC-MS method to investigate the metabolites in mice brain which was used to study the mechanism of context-dependent learning associated with rewarding effect of morphine. Metabolites were extracted from brain tissues and derivatized followed by analysis by gas chromatography/mass spectrometry (GC-MS). In total, 69 peaks were identified as known compounds. By a Wilcoxon ran sum test with p value