A Comprehensive Screening of Escherichia coli Isolates from Scandinavia's Largest Sewage Treatment Plant Indicates No Selection for Antibiotic Resistance
A Comprehensive Screening of Escherichia coli Isolates from Scandinavia's Largest Sewage Treatment Plant Indicates No Selection for Antibiotic Resistance
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DOI：
10.1021/acs.est.8b03354
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发表时间：
2018-10-02
影响因子：
11.4
通讯作者：
Larsson, D. G. Joakim
中科院分区：
文献类型：
--
作者：
Flach, Carl-Fredrik;Genheden, Maja;Larsson, D. G. Joakim
人们担心污水处理厂（STPs）成为耐药细菌出现和选择的热点。然而，通过比较进水和出水中的细菌种群来调查耐药性选择的实地研究产生了不同的，有时甚至是相互矛盾的结果。此外，流入和流出物之间的巨大分类变化使得测量相对基因丰度的研究的解释不明确。这里的目的是通过对从斯堪的纳维亚最大的STP收集的复合进水和出水样本中分离出的大肠杆菌进行全面筛选，并对抗生素残留进行分析，来调查物种内选择是否发生。在18个月内8次收集的4028株菌株对7种抗生素的耐药性进行了筛查。虽然有两次在一些抗生素的流入和流出样本中检测到耐药大肠杆菌的比例存在差异，但随着时间的推移，汇总数据显示，对任何被调查的抗生素都没有这种差异。在处理过程中也没有发现产生多重耐药或广谱β -内酰胺酶的分离株。尽管一些抗生素被检测到的浓度或接近于预测的能够提供一些选择压力的浓度，但对大肠杆菌耐药谱的现场观察并不能为所研究的STP的系统选择提供支持。
There is concern that sewage treatment plants (STPs) serve as hotspots for emergence and selection of antibiotic resistant bacteria. However, field studies investigating resistance selection by comparing bacterial populations in influents and effluents have produced variable and sometimes contradictive results. Also, large taxonomic changes between influents and effluents make interpretation of studies measuring relative gene abundances ambiguous. The aim here was to investigate whether within-species selection occurs by conducting a comprehensive screening of Escherichia coli isolated from composite influent and effluent samples collected at Scandinavia's largest STP, accompanied by analyses of antibiotics residues. In total, 4028 isolates, collected on eight occasions during 18 months, were screened for resistance to seven antibiotics. Although differences in proportions of resistant E. coli between influent and effluent samples were detected for a few antibiotics on two occasions, aggregated data over time showed no such differences for any of the investigated antibiotics. Neither was there any enrichment of multiresistant or extended-spectrum beta-lactamase-producing isolates through the treatment process. Despite some antibiotics were detected at or close to concentrations predicted to provide some selective pressure, field observations of resistance profiles in E. coli do not provide support for systematic selection in the investigated STP.