Spatiotemporal patterns of an evoked network oscillation in neocortical slices: Coupled local oscillators
Spatiotemporal patterns of an evoked network oscillation in neocortical slices: Coupled local oscillators
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DOI：
10.1152/jn.00645.2006
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发表时间：
2006-11-01
影响因子：
2.5
通讯作者：
Wu, Jian-Young
中科院分区：
文献类型：
--
作者：
Bai, Li;Huang, Xiaoying;Wu, Jian-Young
新皮层切片中诱发网络振荡的时空模式：耦合局部振荡。中国生物医学工程学报，26(2):559 - 564。首次发表于2006年7月26日；doi: 10.1152 / jn.00645.2006。我们在大鼠新皮层切片中发现了一个诱发的网络振荡，并用电压敏感染料成像检查了其时空模式。在低钙、高镁的培养基中制备薄片（视觉和听觉皮层），用胆碱代替钠，以减少兴奋毒性和钠负荷。切片后，胆碱被冲洗掉，而钙、镁和钠的浓度恢复正常。通过对浸泡在正常人工脑脊液（ACSF）中的脑切片进行单次电击，可以诱发这种振荡。振荡被组织为一个全有或无的历元，包含4-13个周期，中心频率类似于25赫兹。6-氰基-7-硝基喹啉-2,3-二酮（CNQX）可可逆地阻断其活性。2-氨基-5-磷酸戊酸（APV）和阿托品，但不受双丘碱的影响，提示多突触兴奋机制。电压敏感染料成像显示皮层浅层和中层的高振幅振荡信号。在时空上，振荡以波的形式组织，沿皮层层流水平传播。每一个振荡周期都与一个波在空间中传播有关。在不同的位置（例如，额外的周期）波形往往不同，表明多个局部振荡器共存。对于不同的周期，波浪通常在不同的位置开始，这表明局部振荡子正在竞争启动每个振荡周期。总的来说，我们的研究结果表明，这种皮层网络振荡在两个层面上组织：局部，振荡神经元紧密耦合形成局部振子，全局局部振子之间的耦合很弱，允许突然的空间相位滞后和具有多个起始点的传播波。
Spatiotemporal patterns of an evoked network oscillation in neocortical slices: coupled local oscillators. J Neurophysiol 96: 2528-2538, 2006. First published July 26, 2006; doi:10.1152/jn.00645.2006. We have discovered an evoked network oscillation in rat neocortical slices and have examined its spatiotemporal patterns with voltage-sensitive dye imaging. The slices (visual and auditory cortices) were prepared in a medium of low calcium, high magnesium and with sodium replaced by choline to reduce the excito-toxicity and sodium loading. After slicing, the choline was washed out while normal calcium, magnesium, and sodium concentrations were restored. The oscillation was evoked by a single electrical shock to slices bathed in normal artificial cerebral spinal fluid (ACSF). The oscillation was organized as an all-or-none epoch containing 4-13 cycles at a central frequency similar to 25 Hz. The activity can be reversibly blocked by 6-cyano-7-nitroquinoxalene-2,3- dione (CNQX). 2-amino-5-phosphonopentanoic acid (APV), and atropine but not by bicuculline, indicating polysynaptic excitatory mechanisms. Voltage-sensitive dye imaging showed high-amplitude oscillation signals in superficial and middle cortical layers. Spatio-temporally, the oscillations were organized as waves, propagating horizontally along cortical laminar. Each oscillation cycle was associated with one wave propagating in space. The waveforms were often different at different locations (e.g., extra cycles), suggesting the co-existence of multiple local oscillators. For different cycles, the waves often initiated at different locations, suggesting that local oscillators are competing to initiate each oscillation cycle. Overall our results suggest that this cortical network oscillation is organized at two levels: locally, oscillating neurons are tightly coupled to form local oscillators, and globally the coupling between local oscillators is weak, allowing abrupt spatial phase lags and propagating waves with multiple initiation sites.