Anticariogenic activity of macelignan isolated from Myristica fragrans (nutmeg) against Streptococcus mutans
Anticariogenic activity of macelignan isolated from Myristica fragrans (nutmeg) against Streptococcus mutans
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DOI：
10.1016/j.phymed.2004.04.007
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发表时间：
2006-03-01
期刊：
影响因子：
7.9
通讯作者：
Hwang, J
中科院分区：
文献类型：
--
作者：
Chung, JY;Choo, JH;Hwang, J
龋病的发生主要与口腔致病菌，尤其是致龋性变形链球菌有关。初步抑菌筛选结果表明，广泛种植的肉豆蔻提取物对沙门氏菌有较强的抑制作用。变种人从M.通过1D-NMR、2D-NMR和EI-MS分析，确定其结构为玛克木脂素（macelignan）。变形杆菌的含量为3.9 μ g/ml，远低于其它天然抗致癌剂，如血根碱15.6 μ g/ml、桉叶醇250 μ g/ml、薄荷醇和百里酚500 μ g/ml、水杨酸甲酯1000 μ g/ml。Macelignan还具有对其它口腔微生物的优先活性，例如远缘链球菌、唾液链球菌、血链球菌、嗜酸乳杆菌和干酪乳杆菌，MIC范围为2-31.3 μ g/ml。特别地，杀菌试验表明，在20 μ g/ml的浓度下，macelignan完全灭活S. Macelignan对口腔细菌的特异性活性和快速有效性强烈表明它可以作为功能食品或口腔护理产品中的天然抗菌剂。(c)2005年Elsevier GmbH。All rights reserved.
The occurrence of dental caries is mainly associated with oral pathogens, especially cariogenic Streptococcus mutans. Preliminary antibacterial screening revealed that the extract of Myristica fragrans, widely cultivated for the spice and flavor of foods, possessed strong inhibitory activity against S. mutans. The anticariogenic compound was successfully isolated from the methanol extract of M. fragrans by repeated silica gel chromatography, and its structure was identified as macelignan by instrumental analysis using 1D-NMR, 2D-NMR and EI-MS. The minimum inhibitory concentration (MIC) of macelignan against S. mutans was 3.9 mu g/ml, which was much lower than those of other natural anticarlogenic agents such as 15.6 mu g/ml of sanguinarine, 250 mu g/ml of eucalyptol, 500 mu g/ml of menthol and thymol, and 1000 mu g/ml of methyl salicylate. Macelignan also possessed preferential activity against other oral microorganisms such as Streptococcus sobrinus, Streptococcus salivarius, Streptococcus sanguis, Lactobacillus acidophilus and Lactobacillus casei in the MIC range of 2-31.3 mu g/ml. In particular, the bactericidal test showed that macelignan, at a concentration of 20 mu g/ml, completely inactivated S. mutans in 1 min. The specific activity and fast-effectiveness of macelignan against oral bacteria strongly suggest that it could be employed as a natural antibacterial agent in functional foods or oral care products. (c) 2005 Elsevier GmbH. All rights reserved.