Arabidopsis kinesins HINKEL and TETRASPORE act redundantly to control cell plate expansion during cytokinesis in the male gametophyte
Arabidopsis kinesins HINKEL and TETRASPORE act redundantly to control cell plate expansion during cytokinesis in the male gametophyte
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DOI：
10.1093/mp/ssn042
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发表时间：
2008-09-01
期刊：
影响因子：
27.5
通讯作者：
Twell, David
中科院分区：
文献类型：
--
作者：
Oh, Sung-Aeong;Bourdon, Valerie;Twell, David
花粉有丝分裂I(PMI)的不对称细胞分裂是决定子代营养细胞和生殖细胞的不同命运所必需的。PMI的胞质分裂表现出特殊的特征，有人认为在植物中可能有不同的分子途径来支持不同的胞质分裂模式。NACK-PQR MAP信号通路的激活依赖于NACK1和NACK2激动素相关蛋白，而NACK-PQR是烟草体细胞胞质分裂所必需的。据报道，它们的同源基因Hinkel(HIK)和TETRASPORE(TES)分别对体细胞和小孢子母细胞的胞质分裂是必需的。最近，HIK和TES被证明在雌性配子体胞质分裂中具有功能冗余的作用。我们在这里报道了HIK和TES在小孢子和发育中的花粉中共表达，并通过对双杂合突变体的小孢子和花粉发育的分析，发现在PMI的细胞质分裂过程中发生了细胞板扩张缺陷。这些数据表明，在雄配子体胞质分裂过程中，HIK和TES在细胞板扩张过程中起到了功能上的冗余作用，扩展了不同模式的胞质分裂由共同的信号途径执行的概念，但强化了配子体胞质分裂对TES或HIK的需求的个性。
Asymmetric cell division at pollen mitosis I (PMI) is required to specify the differential fate of the daughter vegetative and generative cells. Cytokinesis at PMI displays specialized features, and it has been suggested that there might be distinct molecular pathways underpinning different modes of cytokinesis in plants. Activation of the NACK-PQR MAP kinase signaling pathway, which is essential for somatic cell cytokinesis in tobacco, depends upon the NACK1 and NACK2 kinesin-related proteins. Their Arabidopsis orthologs, HINKEL (HIK) and TETRASPORE (TES), were reported to be essential for cytokinesis in somatic cells and in microsporocytes, respectively. More recently, HIK and TES were shown to have a functionally redundant role in female gametophytic cytokinesis. We report here that HIK and TES are co-expressed in microspores and developing pollen, and, through analysis of microspore and pollen development in double heterozygote mutants, the occurrence of cell plate expansion defects during cytokinesis at PMI. The data demonstrate a functionally redundant role for HIK and TES in cell plate expansion during male gametophytic cytokinesis, extending the concept that different modes of cytokinesis are executed by a common signaling pathway, but reinforcing the individuality of gametophytic cytokinesis in its requirement for either TES or HIK.