A new cis element is involved in the HER2 gene overexpression in human breast cancer cells.
A new cis element is involved in the HER2 gene overexpression in human breast cancer cells.
复制标题
DOI：
--
复制
发表时间：
1999-06
期刊：
影响因子：
11.2
通讯作者：
M. Grooteclaes;Douglas Vernimmen;Serge Plaza;F. Pasleau;Didier Hodzic;R. Winkler-Gol
中科院分区：
文献类型：
--
作者：
M. Grooteclaes;Douglas Vernimmen;Serge Plaza;F. Pasleau;Didier Hodzic;R. Winkler-Gol
HER2原癌基因产物在30%的乳腺癌中过度表达，这与预后不良有关。乳腺癌细胞系中HER2mRNA水平的升高是由于基因转录增加所致。我们报道了位于转录起始点-506和-489之间的一个新的17bp长的顺式序列的鉴定。该序列被一种反式激活因子识别，我们暂时将其命名为HER2转录因子(HTF)。该因子参与了BT-474乳腺肿瘤细胞中HER2基因转录的增加，其分子量约为50,000。HTF还可以与表皮生长因子受体启动子中存在的相关顺式序列结合，但亲和力较低。有趣的是，在几个过度表达HER2基因的乳腺肿瘤细胞的核提取液中，HTF结合活性很高。
The HER2 proto-oncogene product is overexpressed in 30% of breast cancers, and this correlates with poor prognosis. Increased levels of HER2 mRNA in breast cancer cell lines result from increased gene transcription. We report the identification of a new 17-bp-long cis sequence located between positions -506 and -489 from the transcription start site. This sequence is recognized by a trans-activating factor that we tentatively named HER2 transcription factor (HTF). This factor, involved in the increased transcription of the HER2 gene in the BT-474 mammary tumor cells, has a molecular weight of about Mr 50,000. HTF can also bind, but with a lower affinity, to a related cis sequence present in the epidermal growth factor receptor promoter. Interestingly, the HTF binding activity is high in nuclear extracts from several mammary tumor cells overexpressing the HER2 gene.