Hyaluronic acid hydrogel immobilized with RGD peptides for brain tissue engineering
Hyaluronic acid hydrogel immobilized with RGD peptides for brain tissue engineering
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DOI：
10.1007/s10856-006-0615-7
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发表时间：
2006-12-01
影响因子：
3.7
通讯作者：
Lee, I. -S.
中科院分区：
文献类型：
--
作者：
Cui, F. Z.;Tian, W. M.;Lee, I. -S.
本文以透明质酸水凝胶为材料，制备了具有开放孔结构的脑组织工程支架材料。将精氨酸-甘氨酸-天冬氨酸（RGD）的短肽序列固定在水凝胶的骨架上。将未修饰的水凝胶和用RGD修饰的水凝胶植入大鼠的皮质缺损中，并评价其改善组织重建的能力。6周和12周后，处理脑切片用于DAB和Glees染色。用GFAP和ED 1抗体标记细胞，扫描电镜观察细胞超微结构。植入皮质后，多孔水凝胶作为支架支持细胞浸润和血管生成，同时抑制胶质瘢痕的形成。此外，用RGD修饰的HA水凝胶能够促进神经突延伸。我们的实验表明，透明质酸-RGD水凝胶提供了一个结构，三维连续性的缺陷，有利于重组的局部伤口修复细胞，血管生成和轴突生长到水凝胶支架，而在未改性的水凝胶轴突再生的证据很少。
In this paper, hyaluronic acid hydrogels with open porous structure have been developed for scaffold of brain tissue engineering. A short peptide sequence of arginine-glycine-aspartic acid (RGD) was immobilized on the backbone of the hydrogels. Both unmodified hydrogels and those modified with RGD were implanted into the defects of cortex in rats and evaluated for their ability to improve tissue reconstruction. After 6 and 12 weeks, sections of brains were processed for DAB and Glees staining. They were also labeled with GFAP and ED1 antibodies, and observed under the SEM for ultrastructral examination. After implanting into the lesion of cortex, the porous hydrogels functioned as a scaffold to support cells infiltration and angiogenesis, simultaneously inhibitting the formation of glial scar. In addition, HA hydrogels modified with RGD were able to promote neurites extension. Our experiments showed that the hyaluronic acid-RGD hydrogel provided a structural, three-dimensional continuity across the defect and favoured reorganization of local wound-repair cells, angiogenesis and axonal growth into the hydrogel scaffold, while there was little evidence of axons regeneration in unmodified hydrogel.