CMV antigen-specific CD4+ and CD8+ T cell IFNγ expression and proliferation responses in healthy CMV-seropositive individuals
CMV antigen-specific CD4+ and CD8+ T cell IFNγ expression and proliferation responses in healthy CMV-seropositive individuals
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DOI：
10.1089/0882824041857049
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发表时间：
2004-09-01
期刊：
影响因子：
2.2
通讯作者：
Jacobson, MA
中科院分区：
文献类型：
--
作者：
Sinclair, E;Black, D;Jacobson, MA
在CMV裂解液或CMV pp65或IE肽池刺激后，通过流式细胞术在健康志愿者中测量CMV特异性CD4（+）和CD8(+) T细胞IFNgamma表达和增殖：设置临界值以最大化特异性（即没有假阳性CMV血清阴性）：敏感性（定义为CMV血清阳性对所使用的3种抗原制剂中的至少一种的阳性反应）为100%。CD4（+）和CD8(+) T细胞IFNgamma表达与CD4(+) T细胞增殖及95.4%的cmv特异性CD8(+) T细胞增殖有关。所有22名cmv血清阳性个体在这四项测量中至少有三项有阳性反应。这些发现支持了抗原特异性功能性免疫反应的多样性和病毒特异性CD4(+) T细胞的持久性是这种慢性病毒感染中保护性免疫的重要组成部分的概念。
CMV-specific CD4(+) and CD8(+) T cell IFNgamma expression and proliferation were measured in healthy volunteers by flow cytometry after CMV lysate or CMV pp65 or IE peptide pool stimulation: Cutoff values were set to maximize specificity (i.e., no false positive CMV-seronegatives): Sensitivity (defined as a positive response in CMV-seropositives to at least one of the 3 antigen preparations used) was 100% for CMV-specific. CD4(+) and CD8(+) T cell IFNgamma expression and CD4(+) T cell proliferation and 95.4% for CMV-specific CD8(+) T cell proliferation. All 22 CMV-seropositive individuals had positive responses by at least three of these four measurements. These findings support the concept that a multiplicity of antigen-specific functional immune responses and persistence of robust virus-specific CD4(+) T cells are important components of protective immunity in this chronic viral infection.