Protective Effects and Magnetic Resonance Imaging Temperature Mapping of Systemic and Focal Hypothermia in Cerebral Ischemia
Protective Effects and Magnetic Resonance Imaging Temperature Mapping of Systemic and Focal Hypothermia in Cerebral Ischemia
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DOI：
10.1161/strokeaha.116.014067
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发表时间：
2016-09-01
期刊：
影响因子：
8.3
通讯作者：
Campos, Francisco
中科院分区：
文献类型：
--
作者：
Vieites-Prado, Alba;Iglesias-Rey, Ramon;Campos, Francisco
背景与目的低温治疗是脑缺血最有效的保护性治疗方法，但由于其严重的副作用而限制了其应用。虽然局灶性低温（FH）比全身性低温（SH）具有显著更低的应力分布，但其在缺血中的疗效研究很少。我们的目的是比较各种短期和长期的临床相关endpoint.Methods Sprague-Dawley大鼠短暂（45分钟）大脑中动脉闭塞的治疗效果。动脉再灌注后1小时，将动物随机分配至SH或FH治疗组（目标温度：32 ℃），持续4小时或24小时。在缺血性损伤后1个月评价损伤体积、水肿、功能恢复和细胞损伤的组织学标记。还首次通过磁共振测温法分析了SH和FH对脑温度的影响，这是一种将光谱学与基于梯度回波的相位映射相结合的方法。结果两种治疗方法都减少了缺血性病变体积（P
Background and Purpose Hypothermia is potentially the most effective protective therapy for brain ischemia; however, its use is limited because of serious side effects. Although focal hypothermia (FH) has a significantly lower stress profile than systemic hypothermia (SH), its efficacy in ischemia has been poorly studied. We aimed to compare the therapeutic effects of each treatment on various short- and long-term clinically relevant end points.Methods Sprague-Dawley rats were subjected to transient (45 minutes) occlusion of the middle cerebral artery. One hour after arterial reperfusion, animals were randomly assigned to groups for treatment with SH or FH (target temperature: 32 degrees C) for 4 or 24 hours. Lesion volume, edema, functional recovery, and histological markers of cellular injury were evaluated for 1 month after ischemic injury. Effects of SH and FH on cerebral temperature were also analyzed for the first time by magnetic resonance thermometry, an approach that combines spectroscopy with gradient-echo-based phase mapping.Results Both therapeutic approaches reduced ischemic lesion volume (P