Kinetic Parameters for Pressure−Temperature Inactivation of Bacillus subtilis α‐Amylase under Dynamic Conditions
Kinetic Parameters for Pressure−Temperature Inactivation of Bacillus subtilis α‐Amylase under Dynamic Conditions
复制标题
动态条件下枯草芽孢杆菌 α-淀粉酶压力-温度失活的动力学参数
DOI：
10.1021/bp970053t
复制
发表时间：
1997
期刊：
影响因子：
--
通讯作者：
M. Hendrickx
中科院分区：
文献类型：
--
作者：
L. Ludikhuyze;I. Van den Broeck;C. Weemaes;M. Hendrickx
基于枯草芽孢杆菌α-淀粉酶（BSA）的两种酶模型系统的压力-温度灭活动力学（先前在等压-等温条件下确定）在更现实的（即动态）工艺条件下进行了研究。发现所提出的动力学模型准确描述了在不存在以及存在15%甘油的情况下BSA的非等压/非等温灭活。此外，研究了压力循环对BSA灭活（不存在甘油）的影响。与相同总时间的单次循环过程相比，多次施加压力似乎产生了更显著的灭活效果。然而，这种额外的影响可以完全归因于压力循环期间发生的更广泛的温度变化。
Pressure−temperature inactivation kinetics of two enzymatic model systems based on Bacillus subtilis α‐amylase (BSA), previously determined under isobaric−isothermal conditions, were studied under more realistic, i.e. dynamic, process conditions. The proposed kinetic model was found to accurately describe non‐isobaric/non‐isothermal inactivation of BSA in absence as well as in presence of 15% glycerol. Furthermore, the effect of pressure cycling on the inactivation of BSA (in absence of glycerol) was investigated. Multiple application of pressure seemed to exert a more pronounced inactivation effect as compared to a single‐cycle process of the same total time. This additional effect, however, could be completely attributed to the more extensive temperature variation occurring during pressure cycling.