Synergistic algicidal effect and mechanism of two diketopiperazines produced by Chryseobacterium sp. strain GLY-1106 on the harmful bloom-forming Microcystis aeruginosa.
Synergistic algicidal effect and mechanism of two diketopiperazines produced by Chryseobacterium sp. strain GLY-1106 on the harmful bloom-forming Microcystis aeruginosa.
复制标题
金杆菌GLY-1106产两种二酮哌嗪类药物对水华危害铜绿微囊藻的协同杀藻作用及机制
DOI：
10.1038/srep14720
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发表时间：
2015-10-01
影响因子：
4.6
通讯作者：
Yang H
中科院分区：
文献类型：
--
作者：
Guo X;Liu X;Pan J;Yang H
从太湖中分离到一株高效溶藻菌，命名为黄杆菌GLY-1106，它产生两种溶藻化合物：1106-A（cyclo（4-OH-Pro-Leu））和1106-B（cyclo（Pro-Leu））。两种二酮哌嗪类化合物对太湖水华蓝藻铜绿微囊藻具有较强的杀藻活性。有趣的是，这两种杀藻化合物协同作用。与单独处理相比，cyclo（4-OH-Pro-Leu）和cyclo（Pro-Leu）联合处理显著增强了M.进一步降低了铜绿微囊藻的抗氧化酶活性、有效量子产率和最大电子传递速率。铜绿。cyclo（4-OH-Pro-Leu）和cyclo（Pro-Leu）的溶藻特性也不同。Cyclo（4-OH-Pro-Leu）主要阻断蓝藻光合系统的电子传递，而cyclo（Pro-Leu）主要抑制蓝藻胞内抗氧化酶的活性。根据cyclo（4-OH-Pro-Leu）和cyclo（Pro-Leu）单独和联合处理时的独特杀藻特性，提出了cyclo（4-OH-Pro-Leu）和cyclo（Pro-Leu）协同杀藻的可能机制。这些结果表明，杀藻化合物之间的协同作用可能被用作未来控制微囊藻水华的有效策略。
A potent algicidal bacterium isolated from Lake Taihu, Chryseobacterium sp. strain GLY-1106, produces two algicidal compounds: 1106-A (cyclo(4-OH-Pro-Leu)) and 1106-B (cyclo(Pro-Leu)). Both diketopiperazines showed strong algicidal activities against Microcystis aeruginosa, the dominant bloom-forming cyanobacterium in Lake Taihu. Interestingly, these two algicidal compounds functioned synergistically. Compared with individual treatment, combined treatment with cyclo(4-OH-Pro-Leu) and cyclo(Pro-Leu) significantly enhanced algicidal activity, accelerated the increase in intracellular reactive oxygen species (ROS) levels in M. aeruginosa, and further decreased the activities of antioxidases, effective quantum yield and maximal electron transport rate of M. aeruginosa. The results also showed that the algicidal characteristics of cyclo(4-OH-Pro-Leu) are distinct from those of cyclo(Pro-Leu). Cyclo(4-OH-Pro-Leu) mainly interrupted the flux of electron transport in the cyanobacterial photosynthetic system, whereas cyclo(Pro-Leu) mainly inhibited the activity of cyanobacterial intracellular antioxidases. A possible algicidal mechanism for the synergism between cyclo(4-OH-Pro-Leu) and cyclo(Pro-Leu) is proposed, which is in accordance with their distinct algicidal characteristics in individual and combined treatment. These findings suggest that synergism between algicidal compounds might be used as an effective strategy for the future control of Microcystis blooms.