Aminoglycoside antibiotics and eukaryotic protein synthesis: structure--function relationships in the stimulation of misreading with a wheat embryo system.
Aminoglycoside antibiotics and eukaryotic protein synthesis: structure--function relationships in the stimulation of misreading with a wheat embryo system.
复制标题
氨基糖苷类抗生素和真核蛋白质合成：小麦胚胎系统误读刺激中的结构-功能关系。
DOI：
10.1021/bi00600a001
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发表时间：
1978
期刊：
影响因子：
2.9
通讯作者：
J. Jessop
中科院分区：
文献类型：
--
作者：
J. Wilhelm;S. Pettitt;J. Jessop
材料和方法材料。小麦胚芽来自Niblack Foods，罗切斯特，纽约生物化学品是Sigma的产品。放射性标记的氨基酸（比活性，Ci/mmol）如下获得：[3H]苯丙氨酸（16.1），-脯氨酸（26.6），-酪氨酸（49.5），-缬氨酸（12.5），NEN;[3H]赖氨酸（7），ICN;[3H]精氨酸（0.310），-异亮氨酸（0.306），-亮氨酸（0.303），-丝氨酸（0.162），NEN;[35 S]蛋氨酸（491），NEN; 14 C标记氨基酸混合物（个体为0.102 - 0.420;不含天冬酰胺、半胱氨酸、谷氨酰胺、甲硫氨酸或色氨酸），NEN。抗生素的来源如下：链霉素和新霉素（Sigma）;巴龙霉素、巴龙胺和布替罗新（H. Machamer，Parke，Davis and Co.）;新胺（G.博士）Whitfield，The Upjohn Co.）;利维霉素A和B（Dr. K. Price，布里斯托实验室）;庆大霉素A、B、Bj、Ci、C2和Cía（G. Wagman，Sobering Corp.）;蓝霉素、新霉素B和C、卡那霉素A、B和C（Dr.H.泰伯，罗切斯特大学）。当单独使用时，符号Pm和Nm意味着药物的通常商业形式。对于Pm，这是Pm I和Pm II的混合物。对于Nm，这是NmB和NmC与少量新霉胺（也称为NmA）的混合物。
Materials and Methods Materials. Wheatgerm was from Niblack Foods, Rochester, NY Biochemicals were products of Sigma. Radiolabeled amino acids (specific activity in Ci/mmol) were obtained as follows:[3H] phenylalanine (16.1),-proline (26.6),-tyrosine (49.5),-valine (12.5), NEN;[3H] lysine (7), ICN;[^ argi-nine (0.310),-isoleucine (0.306),-leucine (0.303),-serine (0.162), NEN;[35S] methionine (491), NEN; 14C-labeled amino acid mixture (individual from 0.102 to 0.420; contained no asparagine, cysteine, glutamine, methionine, or tryptophan), NEN. Source of antibiotics was as follows: streptomycin and ne-omycin (Sigma); paromomycin, paromamine, and butirosin (Dr. H. Machamer, Parke, Davis and Co.); neamine (Dr. G. Whitfield, The Upjohn Co.); livídomycins A and B (Dr. K. Price, Bristol Laboratories); gentamicins A, B, Bj, Ci, C2, and Cía (Dr. G. Wagman, Sobering Corp.); bluensomycin, neo-mycins B and C, kanamycins, A, B, and C (Dr. H. Taber, University of Rochester). When used alone, the symbols Pm and Nm imply the usual commercial forms of the drugs. For Pm, this is a mixture of Pm I and Pmll. For Nm, this is a mixture of NmB and NmC with a small amount of neamine (also called NmA).