COORDINATION OF GROWTH WITH CELL-DIVISION IN YEAST SACCHAROMYCES-CEREVISIAE
COORDINATION OF GROWTH WITH CELL-DIVISION IN YEAST SACCHAROMYCES-CEREVISIAE
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DOI：
10.1016/0014-4827(77)90154-9
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发表时间：
1977-01-01
影响因子：
3.7
通讯作者：
HARTWELL, LH
中科院分区：
文献类型：
--
作者：
JOHNSTON, GC;PRINGLE, JR;HARTWELL, LH
酵母细胞的生长和分裂通常是协调的。通过分离这两个过程来研究这种协调的机制。使用温度敏感的细胞分裂周期突变体阻止了分裂，并观察了这种阻止对生长的影响。生长受到营养剥夺的限制，并观察了这种限制对分裂的影响。在细胞周期的各个阶段被阻断的突变体继续生长，细胞体积、质量和蛋白质含量的增加就是证据。启动细胞周期的细胞完成了它们的周期，即使生长受到严重限制，也会在G1期停止。在这些条件下产生的子细胞异常小。这种异常小的细胞在营养恢复后不会启动新的细胞周期(即不会发芽或完成任何已知的3个基因控制步骤（G1中的cdc28， cdc4或cdc7），直到生长到临界大小发生。用适当的交配信息素暂时阻止分裂，形成异常大的细胞；当这些细胞发芽时，当细胞分裂发生时，产生的芽比母细胞小得多。细胞生长与细胞分裂的正常协调可能是以下两种关系的结果：生长，而不是通过dna分裂周期的进展，通常限制细胞增殖的速率；G1中特定的早期事件，在cdc28基因产物控制的事件发生时或之前，直到达到临界大小才能完成。
Yeast cell growth and cell divison are normally coordinated. The mechanism of this coordination was examined by dissociating the 2 processes. Division was arrested with the use of temperature-sensitive cell division cycle mutants, and the effects of this arrest on growth were observed. Growth was limited by nutritional deprivation and the effects of this limitation n division were observed. Mutants blocked at various stages of the cell cycle continued growth, as evidenced by increases in cell volume, mass and protein content. Cells that initiated cell cycles completed their cycles, and arrested in G1 even when growth was severely restricted. Under these conditions the daughter cells produced were abnormally small. Such abnormally small cells did not initiate new cell cycles (i.e., did not bud or complete any of the 3 known gene-controlled steps (cdc28, cdc4 or cdc7 in G1) after nutrients were restored until growth to a critical size occurred. Abnormally large cells were prepared by arresting division temporarily with the appropriate mating pheromone; when such cells were allowed to bud, the buds produced were much smaller than the mother cells when cytokinesis occurred. The normal coordination of cell growth with cell division may be a consequence of the following two relationships: growth, rather than progress through the DNA-division cycle, was normally rate-limiting for cell proliferation; a specific early event in G1, at or before the event controlled by the cdc28 gene product, could not be completed until a critical size was attained.