Action of a GH 51 α-L-arabinofuranosidase on wheat-derived arabinoxylans and arabino-xylooligosaccharides
Action of a GH 51 α-L-arabinofuranosidase on wheat-derived arabinoxylans and arabino-xylooligosaccharides
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DOI：
10.1016/j.carbpol.2007.09.008
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发表时间：
2008-05-16
影响因子：
11.2
通讯作者：
O'Donohue, M.
中科院分区：
文献类型：
--
作者：
Remond, C.;Boukari, I.;O'Donohue, M.
为了准确评价其催化能力，研究了糖苷水解酶51家族中的阿拉伯糖糠糖苷酶（AbfD3）的底物特异性。AbfD3在脱淀粉麦麸上的活性较差，释放的阿拉伯糖总量不到1%。AbfD3还测试了从脱淀粉麦麸中提取的阿拉伯木聚糖，它们具有不同程度的聚合，A/X比，阿魏酸含量和溶解度。结果表明，AbfD3可以水解聚合阿拉伯糖聚糖，即使与低聚糖的高效水解相比，这种作用是温和的。从木聚糖主链上阿拉伯糖残基的不均匀分布、阿拉伯糖木聚糖的不溶性以及存在二取代木糖或阿铁氨酸化阿拉伯糖等方面讨论了AbfD3对聚合物阿拉伯糖木聚糖的有限作用。(C) 2007 Elsevier Ltd.版权所有。
The substrate specificity of an arabinofuranosidase (AbfD3) from family 51 of glycoside hydrolase classification was investigated in order to precisely evaluate its catalytic abilities. AbfD3 activity on destarched wheat bran was poor and less than 1% of total arabinose was released. AbfD3 was also tested on arabinoxylans derived from destarched wheat bran that present different degrees of polymerization, A/X ratios, ferulic acid content and solubility. Results indicated that AbfD3 can hydrolyze polymeric arabinoxylans, even if this action was moderate when compared to the efficient hydrolysis of oligosaccharides. The limited action of AbfD3 on polymeric arabinoxylans is discussed with regard to the heterogeneous distribution of the arabinose residues along the xylan main chain, the insolubility of arabinoxylans and to the presence of disubstituted xylose or feruloylated arabinose. (C) 2007 Elsevier Ltd. All rights reserved.