Low-level sequence variation in Toxoplasma gondii calcium-dependent protein kinases among different genotypes.
Low-level sequence variation in Toxoplasma gondii calcium-dependent protein kinases among different genotypes.
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DOI：
10.4238/2015.may.11.28
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发表时间：
2015-05
期刊：
影响因子：
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通讯作者：
J. Wang;N. Z. Zhang;S. Y. Huang;Y. Xu;R. A. Wang;X. Zhu
中科院分区：
文献类型：
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作者：
J. Wang;N. Z. Zhang;S. Y. Huang;Y. Xu;R. A. Wang;X. Zhu
弓形虫病的病原体，弓形虫，可以感染几乎所有的有核细胞类型的温血动物。在这项研究中，我们研究了钙依赖性蛋白激酶2（CDPK 2）基因的序列变异，13 T。来自不同宿主和地理位置的弓形虫菌株。结果表明，CDPK 2基因的DNA和cDNA全长分别为3671-3673和2136 bp，不同种间的序列变异为0 ~ 0.9%。弓形虫株基于CDPK 2基因序列的系统发育分析表明，T.相同基因型的弓形虫菌株聚在不同的分支中。进一步分析了所有其他T.基因型I（GT 1）、II（ME 49）和III（VEG）的弓形虫CDPK基因表明T.弓形虫CDPK基因家族具有很高的保守性，其序列变异范围为0 ~ 1.40%。我们的结论是，CDPK 2以及T. gondii不能作为研究不同弓形虫变异的合适标记。弓形虫基因型来自不同的宿主和地理位置，但它们的序列保守性可能是促进它们作为抗T.弓形虫疫苗候选人的进一步研究。
The causative agent of toxoplasmosis, Toxoplasma gondii, can infect virtually all nucleated cell types of warm-blooded animals. In this study, we examined the sequence variation in calcium-dependent protein kinase 2 (CDPK2) genes among 13 T. gondii strains from different hosts and geographical locations. The results showed that the lengths of the complete CDPK2 DNA and cDNA sequences were 3671-3673 and 2136 bp, respectively, and the sequence variation was 0-0.9% among different T. gondii strains. Phylogenetic analysis based on the CDPK2 gene sequences revealed that T. gondii strains of the same genotypes were clustered in different clades. Further analysis of all the other T. gondii CDPK genes in genotype I (GT1), II (ME49), or III (VEG) strains indicated the T. gondii CDPK gene family is quite conserved, with sequence variation ranging from 0 to 1.40%. We concluded that CDPK2 as well as all the other CDPK genes in T. gondii cannot be used as proper markers for studying the variants of different T. gondii genotypes from different hosts and geographical locations, but their sequence conservation may be a useful feature promoting them as anti-T. gondii vaccine candidates in further studies.