Lymphatic and Blood Vessel Density in Human Conjunctiva After Glaucoma Filtration Surgery
Lymphatic and Blood Vessel Density in Human Conjunctiva After Glaucoma Filtration Surgery
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DOI：
10.1097/ijg.0000000000000199
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发表时间：
2016-01-01
影响因子：
2
通讯作者：
Edward, Deepak P.
中科院分区：
文献类型：
--
作者：
Bouhenni, Rachida A.;Al Jadaan, Ibrahim;Edward, Deepak P.
目的：研究丝裂霉素C小梁切除术后滤过泡和正常结膜组织中淋巴管微血管密度(LVD)和血管微血管密度(MVD)及其分布。材料与方法：用免疫组织化学方法检测8例正常人结膜组织和8例摘除的滤过泡中淋巴管内皮细胞受体1(D2-40，淋巴管内皮细胞)和CD34(血管内皮细胞)的LVD和MVD。LVD和MVD计数由3名观察者在5个视野、放大倍数为20倍的显微镜下进行。经Mann-Whitney U检验，差异有统计学意义(P<；0.05，P<；0.05)。结果：渗漏的滤过泡呈典型的上皮间质穹隆状，无细胞间质区域被变薄的上皮覆盖，周围为正常结膜上皮和致密的疤痕样基质覆盖。与正常结膜相比，摘除滤过泡的结膜基质中LVD值和MVD值分别为21.42vs.1.16(P<0.002)和24.28vs.1(P<0.008)。与正常结膜标本相比，滤过泡周围基质中的LVD和MVD也降低(2-2.5倍)。结论：在本研究中，低细胞和滤过泡基质中的LCD和MVD降低。这些结果可能表明，这些血管在漏泡的免疫反应改变中发挥了作用，从而增加了患膀胱炎的风险。
Purpose: To investigate the lymphatic vascular microvessel density (LVD) and the blood vascular microvessel density (MVD) and their distribution in excised leaking blebs after mitomycin C trabeculectomy and normal conjunctiva specimens.Materials and Methods: LVD and MVD in normal human conjunctiva (n = 8) and excised blebs in the hypocellular stroma and peribleb tissue (conjunctiva adjacent to hypocellular bleb tissue) (n = 8) were evaluated by immunohistochemistry using antibodies raised against Lymphatic Vessel Endothelial Receptor 1 (D2-40, lymphatic endothelium) and CD34 (vascular endothelium). LVD and MVD counts were performed by light microscopyin 5 fields at X 20 magnification by 3 observers. Differences were determined using Mann-Whitney U test (P < 0.05 was considered significant).Results: The leaking blebs showed typical epithelial-stromal domes with areas of acellular stroma covered by attenuated epithelium and surrounded by normal conjunctival epithelium and a dense scar-like matrix replacing the substantia propria. The LVD and MVD were significantly reduced to nil in the hypocellular conjunctival stroma of the excised blebs compared with normal conjunctiva (21.42 vs. 1.16, P < 0.002 and 24.28 vs. 1, P < 0.008, respectively). The LVD and MVD was also reduced (2- to 2.5-fold) in the peribleb stroma when compared with normal conjunctiva specimens.Conclusions: In this study we show reduced LCD and MVD in the hypocellular and peribleb stroma. These results may suggest a role of these vessels in an altered immune response in leaking blebs leading to an increased risk for blebitis.