Multiphoton endoscopy
Multiphoton endoscopy
复制标题
DOI：
10.1364/ol.28.000902
复制
发表时间：
2003-06-01
期刊：
影响因子：
3.6
通讯作者：
Schnitzer, MJ
中科院分区：
文献类型：
--
作者：
Jung, JC;Schnitzer, MJ
尽管广泛使用的多光子荧光显微镜，内窥镜的非线性光学成像的发展一直受阻的超短激发脉冲，发生在光纤内的群速度色散和自相位调制的组合效应的结果的退化。我们介绍显微内窥镜（直径350-1000微米）的基础上梯度折射率微透镜，有效地消除自相位调制内的内窥镜。激光扫描多光子荧光内窥镜具有微米级的分辨率。我们使用多光子内窥镜对荧光标记的神经元和树突进行成像。(C)2003年美国光学学会。
Despite widespread use of multiphoton fluorescence microscopy, development of endoscopes for nonlinear optical imaging has been stymied by the degradation of ultrashort excitation pulses that occurs within optical fiber as a result of the combined effects of group-velocity dispersion and self-phase modulation. We introduce microendoscopes (350-1000 mum in diameter) based on gradient-index microlenses that effectively eliminate self-phase modulation within the endoscope. Laser-scanning multiphoton fluorescence endoscopy exhibits micrometer-scale resolution. We used multiphoton endoscopes to image fluorescently labeled neurons and dendrites. (C) 2003 Optical Society of America.