Simplex PCR Assay for Positive Identification of Genetic Sex in the Japanese Medaka, Oryzias latipes
Simplex PCR Assay for Positive Identification of Genetic Sex in the Japanese Medaka, Oryzias latipes
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DOI：
10.1007/s10126-008-9106-9
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发表时间：
2008-12-01
影响因子：
3
通讯作者：
Hinze, Susan J.
中科院分区：
文献类型：
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作者：
Patil, Jawahar G.;Hinze, Susan J.
medaka, Oryzias latipes，是生物医学研究中非常受欢迎的模型，特别是在阐明性别分化和内分泌干扰物的决定机制和影响等方面。这些研究需要一种灵敏、准确、快速和可靠的技术来对卵、幼虫和成虫进行遗传性别鉴定。在这项研究中，我们报告了一种单链聚合酶链反应方法，该方法使用一对引物同时扩增性别特异性扩增子。在三种不同的medaka菌株中，雄性和雌性分别产生933和1,906 bp的单一诊断带，允许对未成熟和成年鱼进行准确的性别鉴定。这项技术将在使用medaka和依赖基因性别的生态和生物医学研究中有用。
The medaka, Oryzias latipes, is a very popular model in biomedical research, particularly for elucidating sex differentiation and determination mechanisms and effects of endocrine disruptors among others. These studies require a sensitive, accurate, rapid, and reliable technique for genetic sexing of eggs, larvae, and adults. In this study, we report a simplex polymerase chain reaction approach that uses a single pair of primers for simultaneous amplification of sex-specific amplicons. Males and females yield a single diagnostic band of 933 and 1,906 bp, respectively, in three different strains of medaka tested, permitting gender identification accurately of both immature and adult fish. This technique will be useful in both ecological and biomedical researches that employ medaka and rely on genetic sexing.