Current progress of genome editing techniques
Current progress of genome editing techniques
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发表时间：
2015
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通讯作者：
Ma Cha
中科院分区：
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作者：
Ma Cha
基因组编辑是一种针对目标基因组的定点修饰技术，其主要方法是对目标基因组进行修饰，以研究功能未知的基因并进行基因治疗。人工核酸内切酶介导的基因组编辑技术已发展成为目前最有前途的技术，包括ZFNs、TALENs和CRISPR/Cas技术。本文简要介绍了三种不同原理的基因组编辑技术，比较了它们的优缺点。此外，总结了不同生物基因组编辑技术的研究现状，探索其在动物疾病模型中的应用，并推测该技术在疾病基因治疗中的应用前景。
Genome editing is a site-directed modification technology for target genome, whose major method is to modify target genome for the purpose of studying the gene with unknown functions and conducting gene therapy. The genome editing technology mediated by artificial endonuclease have evolved into the most promising technology at present, including ZFNs、TALENs and CRISPR/Cas technologies. In this paper, a brief introduction is given to three different principles of genome editing technologies to compare their advantages and disadvantages. In addition, the research status concerning the editing technologies for the genomes of different creatures has been summarized to explore its application in animal disease model and speculate on the application prospect of the technology in gene therapy of diseases.