A 'catch-and-release' receptor for the cholera toxin.
A 'catch-and-release' receptor for the cholera toxin.
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DOI：
10.1039/c9fd00017h
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发表时间：
2019-03
影响因子：
3.4
通讯作者：
Clare S. Mahon;Clare S. Mahon;G. Wildsmith;Diksha Haksar;E. D. Poel;J. Beekman;R. Pieters;Michael E Webb;W. B. Turnbull;W. B. Turnbull
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文献类型：
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作者：
Clare S. Mahon;Clare S. Mahon;G. Wildsmith;Diksha Haksar;E. D. Poel;J. Beekman;R. Pieters;Michael E Webb;W. B. Turnbull;W. B. Turnbull
霍乱毒素（CTB）识别单元的刺激响应性受体已通过将其天然碳水化合物配体GM1寡糖的多个拷贝连接到温敏聚合物支架上来制备。低于它们的低临界溶解温度（LCST），聚合物以纳摩尔亲和力络合CTB。当加热到高于其LCST时，聚合物经历可逆的卷曲到球转变，这使得一部分碳水化合物识别基序无法被CTB接近。这种热调节的材料对蛋白质的亲合力的降低已被用于从溶液中可逆地捕获CTB，使得能够从复杂混合物中方便地分离CTB。
Stimuli-responsive receptors for the recognition unit of the cholera toxin (CTB) have been prepared by attaching multiple copies of its natural carbohydrate ligand, the GM1 oligosaccharide, to a thermoresponsive polymer scaffold. Below their lower critical solution temperature (LCST), polymers complex CTB with nanomolar affinity. When heated above their LCST, polymers undergo a reversible coil to globule transition which renders a proportion of the carbohydrate recognition motifs inaccessible to CTB. This thermally-modulated decrease in the avidity of the material for the protein has been used to reversibly capture CTB from solution, enabling its convenient isolation from a complex mixture.