Selection of anionic dopant for quantifying desialylation reactions with MALDI-FTMS.
Selection of anionic dopant for quantifying desialylation reactions with MALDI-FTMS.
复制标题
选择阴离子掺杂剂，用 MALDI-FTMS 定量脱唾液酸化反应。
DOI：
10.1021/ac990956w
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发表时间：
2000
影响因子：
7.4
通讯作者：
Lebrilla,CB
中科院分区：
文献类型：
--
作者：
Wong,AW;Wang,H;Lebrilla,CB
研究了烷基磺酸盐作为中性寡糖基质辅助激光解吸/电离质谱（MALDI-MS）阴离子掺杂剂的作用。阴离子掺杂剂允许在与酸性寡糖相同的混合物中检测中性寡糖。烷基磺酸盐掺杂剂与寡糖强烈相互作用，产生由寡糖和去质子化烷基磺酸盐组成的准分子离子。小到二糖，大到支链寡糖的化合物都可以检测。在负离子模式下，新系列的烷基磺酸盐在信噪比、灵敏度和易于制备方面与HSO 4-一样有效。烷基磺酸盐用于构建校准曲线，以获得不同中性和酸性寡糖混合物的相对量。与阴离子掺杂剂，MALDI-FTMS信号显示具有线性关系的中性和唾液酸化寡糖的摩尔比。该方法还用于监测通过酸水解的去唾液酸化反应的产物。
Alkylsulfonates are examined as anion dopants for the matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) of neutral oligosaccharides. The anion dopants allow neutral oligosaccharides to be examined in the same mixture as acidic oligosaccharides. The alkylsulfonate dopants interact strongly with the oligosaccharide to produce a quasimolecular ion composed of the oligosaccharides and the deprotonated alkylsulfonates. Compounds as small as disaccharides to large branched oligosaccharides can all be examined. The new series of alkylsulfonates works as well as HSO4-in terms of signal-to-noise ratio, sensitivity, and ease of preparation in the negative ion mode. The alkylsulfonates are used to construct a calibration curve for obtaining the relative amounts of varying neutral and acidic oligosaccharide mixtures. With the anion dopants, the MALDI-FTMS signals are shown to have a linear relationship with the molar ratios of the neutral and sialylated oligosaccharides. The method is also used to monitor the product of a desialylation reaction by acid hydrolysis.