Proteomic analysis of Theobroma cacao pod husk.
Proteomic analysis of Theobroma cacao pod husk.
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DOI：
10.5458/jag.57.245
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发表时间：
2010-01-01
影响因子：
1.1
通讯作者：
Mitsui, T.
中科院分区：
文献类型：
--
作者：
Azwan Awang, Azwan Awang;Rafiah Karim, Rafiah Karim;Mitsui, T.
我们对可可豆荚壳（果皮）进行了二维电泳 (2-DE) 和 MS 分析，以探索这种顽固组织的蛋白质组。使用苯酚提取/甲醇-醋酸铵沉淀方法，我们获得了 2-DE 图像，在胶体 CBB 染色后检测到约 700 个蛋白点。通过 MALDI-TOF/TOF MS 对 SPITC 衍生的胰蛋白酶肽进行从头测序，分析了 244 个蛋白质点。应用这项技术，从可可豆荚壳中鉴定出了 144 个蛋白质点。大多数已识别的蛋白质与新陈代谢和能量有关。其中一些蛋白质可能与豆荚的生长和发育过程有关。
We performed two dimensional electrophoresis (2-DE) coupled with MS analysis on Theobroma cacao pod husk (fruit pericarp) to explore the proteome of this recalcitrant tissue. Using a phenol extraction/methanol-ammonium acetate precipitation method, we have obtained 2-DE images with approximately 700 protein spots detected after colloidal CBB staining. Two hundred and forty-four protein spots were analyzed by de novo sequencing of SPITC-derivatized tryptic peptides by MALDI-TOF/TOF MS. Applying this technique, 144 protein spots from cocoa pod husk were identified. The majority of the identified proteins were involved in metabolism and energy. Several of these proteins could be linked to pod growth and development processes.