A new stable and reliable method for labeling nucleic acids of fully replicative viruses.
A new stable and reliable method for labeling nucleic acids of fully replicative viruses.
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DOI：
10.1039/c2cc17069h
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发表时间：
2012-02
影响因子：
4.9
通讯作者：
Lili Huang;Peng Zhou;Han-zhong Wang;Rui Zhang;Jian-wei Hao;Hai‐Yan Xie;Zhike He
中科院分区：
文献类型：
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作者：
Lili Huang;Peng Zhou;Han-zhong Wang;Rui Zhang;Jian-wei Hao;Hai‐Yan Xie;Zhike He
有效标记完全复制病毒的核酸是一个挑战。在这项工作中，“分子光开关”复合物 [Ru(phen)(2)(dppz)](2+)（其中 phen = 1,10-菲咯啉，dppz = 二吡啶并[3,2-a:2',3'-c]吩嗪）已被探索性地用于标记痘苗病毒核酸。标记的病毒粒子表现出强烈且稳定的荧光，并且可以在单个病毒粒子水平上成像。此外，它们具有完全的传染性，可用于研究入侵宿主细胞的行为。该方法通用，适合标记各种DNA病毒。
Efficiently labeling nucleic acids of fully replicative viruses is a challenge. In this work, a 'molecular light switch' complex [Ru(phen)(2)(dppz)](2+), where phen = 1,10-phenanthroline and dppz = dipyrido[3,2-a:2',3'-c]phenazine, has been exploringly used to label vaccinia virus nucleic acid. The labeled virions exhibited strong and stable fluorescence and could be imaged at the single-virion level. Moreover, they were fully infectious and can be used to study the behaviors of invasion into their host cells. The method is general and suitable for labeling various DNA viruses.