Sicilian semi- and supercentenarians: identification of age-related T-cell immunophenotype to define longevity trait
Sicilian semi- and supercentenarians: identification of age-related T-cell immunophenotype to define longevity trait
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DOI：
10.1093/cei/uxad074
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发表时间：
2023-12-11
影响因子：
4.6
通讯作者：
Candore，Giuseppina
中科院分区：
文献类型：
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作者：
Ligotti，Mattia Emanuela;Accardi，Giulia;Candore，Giuseppina
最年长的百岁老人，即半百岁老人和超百岁老人的免疫表型可以提供有关其适应与免疫变化相关因素的能力的重要信息，包括衰老本身和慢性巨细胞病毒感染。我们通过流式细胞术研究了28名女性和26名男性（年龄范围19-110岁）队列中免疫细胞亚群的百分比和绝对数量的变化，重点是T细胞和促炎参数。我们观察到与年龄和巨细胞病毒血清学状态相关的免疫衰老标志的变异性。8名年龄最大的百岁老人由于其年龄而显示出幼稚T细胞的最低百分比，根据其巨细胞病毒状态，T效应记忆细胞重新表达CD 45 RA（TEMRA）的百分比最高，血清促炎参数水平较高，尽管他们的平均值低于其余90+供体。他们中的一些人显示出CD 8幼稚和TEMRA百分比，以及与年轻人相当的疲惫/促炎标志物。我们的研究支持这样的建议，即免疫衰老，特别是最古老的百岁老人，表现出很大的变化，这不仅是由于一个单一的贡献者，但也应该是几个因素的组合的全部结果。每个人的年龄都不同，因为他/她在遗传和生活经验方面是独一无二的，这更适用于免疫系统;每个人都有不同的免疫史。此外，我们的研究结果炎症标志物，TEMRA和CMV血清阳性的百岁老人，根据最近的文献进行了讨论，表明这些变化可能不是不利的百岁老人，特别是最古老的。
The immunophenotype of oldest centenarians, i.e. semi- and supercentenarians, could provide important information about their ability to adapt to factors associated with immune changes, including ageing per se and chronic Cytomegalovirus infection. We investigated, by flow cytometry, variations in percentages and absolute numbers of immune cell subsets, focusing on T cells, and pro-inflammatory parameters in a cohort of 28 women and 26 men (age range 19–110 years). We observed variability in hallmarks of immunosenescence related to age and Cytomegalovirus serological status. The eight oldest centenarians showed the lowest percentages of naïve T cells, due to their age, and the highest percentages of T-effector memory cells re-expressing CD45RA (TEMRA), according to their cytomegalovirus status, and high levels of serum pro-inflammatory parameters, although their means were lower than that of remaining 90+ donors. Some of them showed CD8 naïve and TEMRA percentages, and exhaustion/pro-inflammatory markers comparable to the younger ones. Our study supports the suggestion that immune ageing, especially of oldest centenarians, exhibits great variability that is not only attributable to a single contributor but should also be the full result of a combination of several factors. Everyone ages differently because he/she is unique in genetics and experience of life and this applies even more to the immune system; everybody has had a different immunological history. Furthermore, our findings on inflammatory markers, TEMRA and CMV seropositivity in centenarians, discussed in the light of the most recent literature, suggest that these changes might be not unfavourable for centenarians, and in particular for the oldest ones.