Effects of aqueous extracts of Taraxacum Officinale on expression of tumor necrosis factor-alpha and intracellular adhesion molecule 1 in LPS-stimulated RMMVECs.
Effects of aqueous extracts of Taraxacum Officinale on expression of tumor necrosis factor-alpha and intracellular adhesion molecule 1 in LPS-stimulated RMMVECs.
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DOI：
10.1186/s12906-016-1520-3
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发表时间：
2017-01-11
影响因子：
--
通讯作者：
Yang Z
中科院分区：
文献类型：
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作者：
Hu G;Wang J;Hong D;Zhang T;Duan H;Mu X;Yang Z
乳腺炎给农业（主要是乳品生产）和公共卫生带来了巨大的经济负担。其发病率目前很高，因此，需要高度有效的治疗方法，特别是天然产品。蒲公英已被报道用于抗炎。然而，其在乳腺炎期间对内皮的影响尚未报道。首次建立了大鼠乳腺微血管内皮细胞（RMMVECs）炎症实验模型。我们通过酶联免疫吸附试验和Western印迹法评价了竹叶水提取物（DAE）对LPS诱导的RMMVEC炎症介质产生的影响。用1 μg/ml LPS处理RMMVEC 4 h，然后分别用10、100和200 μg/mL DAE孵育4、8、12和24 h。采用实时定量PCR和Western印迹分析靶点（肿瘤坏死因子-α（TNF- α）和细胞内粘附分子1（ICAM 1））的表达（mRNA和蛋白水平）。还测定了DAE对金黄色葡萄球菌诱导的小鼠模型中乳腺炎的体内抗炎作用。结果表明，黄芪提取物在100和200 μg/mL浓度下可显著抑制TNF-α和ICAM-1的表达，而黄芪提取物在10 μg/mL浓度下仅在处理后8和12 h抑制TNF-α和ICAM-1的表达。体内实验表明，DAE可抑制TNF-α和ICAM-1的表达，并呈时间依赖性。结果提示，内皮细胞有可能作为抗炎作用的靶点。
Mastitis gives rise to big financial burden to farm industry (mainly dairy production) and public health. Its incidence is currently high and therefore, highly effective treatments for therapy, especially with natural products are required. Taraxacum officinale has been reported to use for anti-inflammation. However, its effect on endothelium during mastitis has not been reported. We firstly established inflammation experimental model of rat mammary microvascular endothelial cells (RMMVECs). We evaluated the effects of dandelion leaf aqueous extracts (DAE) on LPS-induced production of inflammatory mediators in RMMVECs by enzyme-linked immunosorbent assay and Western blot. We treated RMMVECs with 1 μg/ml LPS for 4 h and then incubated with 10, 100 and 200 μg/mL DAE for 4, 8, 12 and 24 h. The expression (mRNA and protein level) of targets (tumor necrosis factor-alpha (TNF- α) and Intracellular Adhesion Molecule 1 (ICAM1) was analyzed by employing real-time PCR and Western blots. The in vivo anti-inflammatory effect of DAE on mastitis within an Staphylococcus aureus-induced mouse model was also determined. The obtained results showed that dandelion extracts at the concentration of 100 and 200 μg/mL could significantly inhibit both TNF-α and ICAM-1 expression in all time points checked while 10 μg/mL of dandelion only suppress both expression at 8 and 12 h post-treatment. The in vivo tests showed that the DAE inhibited the expression of TNF-α and ICAM-1 in a time-dependent manner. All results suggest that the endothelium may use as as a possible target of dandelion for anti-inflammation.