Type V collagen is increased during rabbit medial collateral ligament healing
Type V collagen is increased during rabbit medial collateral ligament healing
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DOI：
10.1007/s001670000134
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发表时间：
2000-09-01
影响因子：
3.8
通讯作者：
Woo, SLY
中科院分区：
文献类型：
--
作者：
Niyibizi, C;Kavalkovich, K;Woo, SLY
为了了解内侧副韧带（MCL）的修复过程，分析了前交叉韧带（ACL）重建修复MCL时的纤维状胶原及其相对比例。骨骼成熟的新西兰白兔遭受 MCL 拖把端撕裂，未用 ACL 重建进行修复。受伤后 6 周和 52 周处死兔子。将损伤部位的韧带组织和假手术对照浸泡在 0.5 M 乙酸中 24 小时，切碎，并用胃蛋白酶处理以溶解胶原蛋白。根据胃蛋白酶残留物的羟脯氨酸分析测定，胃蛋白酶溶解了约 80% 的总胶原蛋白。对溶解的胶原蛋白进行十二烷基硫酸钠聚丙烯酰胺凝胶电泳分析显示存在 I、III 型纤维状胶原蛋白。与I型、III型和V型胶原相对应的蛋白质条带的光密度扫描表明，在假手术对照中，III型和V型胶原分别占I型胶原的约8%和12%，而在6周时愈合的MCL韧带显示In型和V型胶原显着增加，分别达到约19%和24%。到 52 周时，愈合的 MCL 中的 III 型胶原蛋白已恢复到假手术对照组的水平，而 V 型胶原蛋白仍保持在约 18% 的升高水平。这些数据表明，愈合韧带中高浓度 V 型胶原的存在可能会对愈合韧带中看到的胶原纤维直径产生影响，在评估韧带愈合时应将其纳入分析中。
To understand the reparative process of medial collateral ligament (MCL), fibrillar collagen and their relative ratios in healing MCL with anterior cruciate ligament (ACL) reconstruction were analyzed. Skeletally mature New Zealand white rabbits were subjected to a mop-end tear of MCL without repair with ACL reconstruction Rabbits were killed 6 and 52 weeks after injury. Ligamentous tissues from the injury site and sham controls were soaked in 0.5 M acetic acid for 24 h, minced, and treated with pepsin to solubilize collagen. Pepsin solubilized about 80% of the total collagen as determined by hydroxyproline analysis of the pepsin residues. Sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis of the solubilized collagen revealed presence of fibrillar collagen types I, III. and V. Densitometric scanning of the protein bands corresponding to types I, III, and V collagen indicated that in sham controls types III and V collagen represented about 8% and 12%, respectively, of the type I collagen whereas the healed MCL ligaments at 6 weeks showed significant increase in type In and V collagen to about 19% and 24%, respectively. By 52 weeks type III collagen in the healed MCL had returned to that of sham controls while type V collagen remained elevated at approximately 18%. These data suggest that presence of type V collagen in high concentration in healing ligaments may have an influence on collagen fibril diameters seen in healed ligament and should be included in the analysis when evaluating ligament healing.