A distal nephron glycoprotein that has different cell surface distributions on MDCK cell sublines.
A distal nephron glycoprotein that has different cell surface distributions on MDCK cell sublines.
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一种远端肾单位糖蛋白，在 MDCK 细胞亚系上具有不同的细胞表面分布。
DOI：
10.1152/ajpcell.1987.253.3.c433
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Herz,RE
中科院分区：
文献类型：
--
作者：
Ojakian,GK;Romain,RE;Herz,RE
识别MDCK细胞质膜上23 kDa糖蛋白（gp 23）的三个不同表位的单克隆抗体用于研究细胞表面极性。从美国典型培养物保藏中心获得的MDCK细胞的免疫荧光显微镜检查表明，gp 23在约50%的细胞中是非极化的（在顶膜和基底外侧膜上），而在其余的细胞中，gp 23具有极化分布（仅基底外侧）。这种异质性的gp 23细胞表面染色未观察到在各种其他MDCK亚系。相反，发现gp 23在MDCK细胞上具有非极化分布，其产生具有低跨上皮电阻（小于220 Ω X cm 2）的单层，并且仅定位于能够产生相当高的跨上皮电阻（770- 2，220 Ω X cm 2）的MDCK细胞系的基底外侧膜。狗，大鼠和兔肾脏的免疫荧光和免疫电镜显示，gp 23是一个肾单位段特异性糖蛋白，仅定位于远端和集合小管。这些观察结果为MDCK细胞系的异质性提供了进一步的证据。他们还支持MDCK细胞起源于肾远端肾单位的观点。
Monoclonal antibodies that recognize three distinct epitopes of a 23-kDa glycoprotein (gp23) on the plasma membrane of MDCK cells were used to study cell-surface polarity. Immunofluorescence microscopy of MDCK cells obtained from the American Type Culture Collection demonstrated that gp23 was nonpolarized in approximately 50% of the cells (on both apical and basolateral membranes), whereas, in the remaining cells, gp23 had a polarized distribution (basolateral only). This heterogeneous gp23 cell-surface staining was not observed in a variety of other MDCK sublines. Instead, gp23 was found to have a nonpolarized distribution on MDCK cells that produced monolayers with low transepithelial electrical resistances (less than 220 omega X cm2) and was localized only to the basolateral membrane of MDCK cell lines capable of generating considerably higher transepithelial electrical resistances (770-2,220 omega X cm2). Immunofluorescence and immunoelectron microscopy of dog, rat, and rabbit kidney demonstrated that gp23 is a nephron segment-specific glycoprotein localized only to the distal and collecting tubules. These observations provide further evidence for the heterogeneity of the MDCK cell line. They also support a proposal that the origin of MDCK cells is the renal distal nephron.