Development of a simple IgE-independent anaphylactic model using buckwheat antigen and B-type CpG oligodeoxynucleotide from Streptococcus thermophilus
Development of a simple IgE-independent anaphylactic model using buckwheat antigen and B-type CpG oligodeoxynucleotide from Streptococcus thermophilus
复制标题
使用荞麦抗原和来自嗜热链球菌的 B 型 CpG 寡脱氧核苷酸开发简单的不依赖 IgE 的过敏模型
DOI：
10.1111/asj.12479
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发表时间：
2016
影响因子：
2
通讯作者：
Shimosato T
中科院分区：
文献类型：
--
作者：
Yamamoto Y;Shigemori S;Nigar S;Oshiro K;Wang Y;Sato T;Shimosato T
我们用荞麦抗原和来自嗜热链球菌基因组的B-型CpG-寡脱氧核苷酸（CpG-ODNs）建立了小鼠严重过敏模型。在典型的全身性过敏反应模型中，通过静脉内（i. v.）路线在这里，我们显示了通过腹膜内（i. p.）路线腹腔注射方法比静脉注射给药简单，失败的风险较低。为了产生该过敏性模型，首先用与B-型CpG-ODN混合的荞麦抗原腹膜内致敏5周龄雌性BALB B/c小鼠。2周后，用抗原激发小鼠以诱导过敏性休克，通过对严重症状进行评分并测量各种蛋白质和脾细胞产生细胞因子的血清水平来评估。抗原与B-型CpG-ODN混合免疫小鼠，其免疫球蛋白（IG）G2 a生成和γ-干扰素阳性细胞显著增加，而血清IgE水平则被B-型CpG-ODN降低。我们还检查了各种ODN（A，B和C-型CpG-ODN）和抗原（荞麦，α-酪蛋白，β-乳球蛋白和卵清蛋白）对过敏严重程度的影响，发现荞麦和B-型CpG-ODN的组合诱导了最强烈的过敏性休克。该模型有望为过敏性休克的预防研究做出贡献。
We developed a severe anaphylactic model in mice using buckwheat antigen and B‐type CpG‐oligodeoxynucleotides (CpG‐ODNs) fromStreptococcus thermophilusgenome. In typical systemic anaphylaxis models, animals are challenged with large quantity of antigens via an intravenous (i.v.) route. Here, we showed a simple anaphylactic shock after challenge via intraperitoneal (i.p.) route. The i.p. method is simpler than i.v. administration and has a lower risk for failure. To generate this anaphylactic model, 5‐week‐old female BALB/c mice were first i.p. sensitized with buckwheat antigen mixed with B‐type CpG‐ODN. After 2 weeks, mice were challenged with antigen to induce anaphylactic shock, which was evaluated by scoring the severity symptoms and measuring serum levels of various proteins and splenic cell producing cytokines. Immunoglobulin (Ig)G2aproduction and interferon‐γ positive cells were markedly increased in mice immunized with antigen mixed with B‐type CpG‐ODN, whereas serum IgE levels were decreased by B‐type CpG‐ODN. We also examined the effects of various ODNs (A, B and C‐type CpG‐ODNs) and antigens (buckwheat, α‐casein, β‐lactoglobulin and ovalbumin) on anaphylactic severity, and found that the combination of buckwheat and B‐type CpG‐ODN induced the most intense anaphylactic shock. This model is expected to contribute to the study of the prevention of anaphylactic shock.