Bacteria known to induce settlement of larvae of Hydroides elegans are rare in natural inductive biofilm
Bacteria known to induce settlement of larvae of Hydroides elegans are rare in natural inductive biofilm
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DOI：
10.3354/ame01925
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发表时间：
2020-01-01
影响因子：
1.4
通讯作者：
Hadfield, Michael G.
中科院分区：
文献类型：
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作者：
Vijayan, Nidhi;Hadfield, Michael G.
像许多底栖海洋无脊椎动物一样，多毛类Hydroides elegans的幼虫在野外对复杂的多物种生物膜和实验室中对某些单特异性细菌生物膜作出反应。本研究使用高通量16 S rRNA-扩增子测序表征了来自美国夏威夷珍珠港的复杂天然生物膜的细菌多样性，并进一步表征了可以使用标准培养方法分离的那些细菌物种。生物膜的群落分析揭示了拟杆菌属、疣微杆菌属、α-和γ-变形菌属的优势，在较低的分类水平上，丝单胞菌属以及鞘氨醇单胞菌科和杜鹃花科的成员。这些天然生物膜诱导了近100%的H.优雅的人暴露在他们面前。我们从标准化数据集中鉴定了田间生物膜中1636种不同的扩增子序列变体。使用一种培养基类型的基于培养的方法仅产生40个分离株，其中36个基于97%的序列相似性截止值是独特的。经鉴定，分离物分属于11个属。在40株假交替单胞菌中，只有6株菌株的单种生物膜能诱导H.优雅然而，假交替单胞菌以非常低的相对丰度存在（
Like those of many benthic marine invertebrates, larvae of the polychaete Hydroides elegans settle in response to complex, multi-species biofilms in the field and to certain monospecific bacterial biofilms in the laboratory. This study characterized the bacterial diversity of complex natural biofilms from Pearl Harbor, Hawai'i, USA, using high-throughput 16S rRNA-amplicon sequencing and further characterized those bacterial species that could be isolated using standard culture methods. Community analysis of the biofilms revealed dominance of Bacteroidetes, Verrucomicrobiae, Alpha- and Gammaproteobacteria and, at lower taxonomic levels, Rubritalea spp., Hyphomonas spp. and members of Sphingomonadaceae and Rhodobacteraceae. These natural biofilms induced settlement in nearly 100% of larvae of H. elegans exposed to them. We identified 1636 different amplicon sequence variants in the field biofilm from the normalized data set. The culture-based methods with one media type produced only 40 isolates, of which 36 were unique based on 97 % sequence similarity cut-off. The cultured isolates were assigned to 11 genera. Single-species biofilms of only 6 of the 40 isolates, all in the genus Pseudoalteromonas, induced settlement by larvae of H. elegans. However, Pseudoalteromonas was present at very low relative abundance (