Scaffoldless Tissue-engineered Dental Pulp Cell Constructs for Endodontic Therapy
Scaffoldless Tissue-engineered Dental Pulp Cell Constructs for Endodontic Therapy
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DOI：
10.1177/0022034513517901
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发表时间：
2014-03-01
影响因子：
7.6
通讯作者：
Sfeir, C.
中科院分区：
文献类型：
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作者：
Syed-Picard, F. N.;Ray, H. L., Jr.;Sfeir, C.
根管治疗后根尖周炎的一个主要原因是细菌的渗透，而细菌的渗透可能会受到活髓的存在的挑战。在这项研究中，自组装，无支架，三维(3D)组织工程从牙髓细胞(DPC)和评估作为一种设备的牙髓再生。这些工程组织被放置到人类牙根段的根管间隙中，其中一端用磷酸钙骨水泥覆盖，整个系统被植入小鼠的皮下。组织学染色显示，种植3个月和5个月后，含有3D无支架工程组织的牙根始终保持细胞和纤维组织，而空牙根以空牙根为主。免疫组织化学染色显示，在含有无支架DPC工程组织的根管中发现的组织是血管的，CD31的表达为特征，牙本质唾液蛋白的免疫染色结果表明，根管中含有沿根壁排列的成牙本质细胞样细胞。这项研究表明，三维自组装无支架DPC工程组织可以在牙根管系统中再生出重要的牙髓样组织，因此在牙髓治疗中具有广阔的应用前景。
A major cause of apical periodontitis after endodontic treatment is the bacterial infiltration which could have been challenged by the presence of a vital pulp. In this study, self-assembled, scaffoldless, three-dimensional (3D) tissues were engineered from dental pulp cells (DPCs) and assessed as a device for pulp regeneration. These engineered tissues were placed into the canal space of human tooth root segments that were capped on one end with calcium phosphate cement, and the entire system was implanted subcutaneously into mice. Histological staining indicated that after three- and five-month implantations, tooth roots containing 3D scaffoldless engineered tissues maintained a cellular, fibrous tissue throughout, whereas empty tooth roots remained predominantly empty. Immunostaining indicated that the tissue found in the root canals containing scaffoldless DPC engineered tissues was vascular, as characterized by the expression of CD31, and contained odontoblast-like cells organized along the length of the root wall as assessed by immunostaining for dentin sialoprotein. This study shows that 3D self-assembled scaffoldless DPC engineered tissues can regenerate a vital dental pulp-like tissue in a tooth root canal system and are therefore promising for endodontic therapy.