Rapid Determination of 6-Methyl-5-hepten-2-one in Fruit with LLE-GC-MS
Rapid Determination of 6-Methyl-5-hepten-2-one in Fruit with LLE-GC-MS
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DOI：
10.1093/chromsci/bmab071
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发表时间：
2021-06-30
影响因子：
1.3
通讯作者：
Wang, Haijing
中科院分区：
文献类型：
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作者：
Zhou, Zhen;Wang, Xiuqing;Wang, Haijing
挥发性化合物6-甲基-5-庚烯-2-酮（MHO）是类胡萝卜素在水果代谢过程中产生的重要化学中间体和风味成分，是影响消费者喜爱度的重要因素。果实基质复杂，在MHO分析中纯化难度较大。建立了液相萃取-气相色谱-质谱联用（le - gc - ms）快速测定水果中MHO含量的方法。内标法测定的MHO在100 ~ 2000 ng/ml范围内线性良好。检测限（LOD）和定量限（LOQ）均为
The volatile compound 6-methyl-5-hepten-2-one (MHO) is an important chemical intermediate and flavor component derived from carotenoid during fruit metabolism, which is important factor impacting consumer-liking. Fruit has complex matrix and is difficult to be purified during MHO analysis. A rapid liquid-liquid extraction-gas chromatography-mass spectrometry (LLE-GC-MS) method was developed for effective determination of MHO in fruit in this study. The calibration curves for MHO showed high linearity in the range of 100-2000 ng/ml with internal standard method. The limit of detection (LOD), limit of quantification (LOQ) were both