ON THE NATURE OF A DEFECT IN CELLS FROM INDIVIDUALS WITH ATAXIA-TELANGIECTASIA
ON THE NATURE OF A DEFECT IN CELLS FROM INDIVIDUALS WITH ATAXIA-TELANGIECTASIA
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DOI：
10.1126/science.3975628
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发表时间：
1985-01-01
期刊：
影响因子：
56.9
通讯作者：
BEDFORD, JS
中科院分区：
文献类型：
--
作者：
CORNFORTH, MN;BEDFORD, JS
共济失调-毛细血管扩张症(A-T)是一种遗传性疾病，其特点是高度倾向于癌症，患者的细胞和组织对电离辐射异常敏感。对正常人和A-T成纤维细胞的非周期培养进行X射线照射，以比较过早凝聚的染色体在G1期的断裂和重新连接。在剂量为6.0灰色后，两种细胞类型具有相同的初始断裂频率和相同的断裂再接合率，但A-T细胞未再结合的断裂比例是A-T细胞的5-6倍。细胞进入S期并不是照射处于G1或G0期的A-T细胞后，有丝分裂中出现的染色体片段频率增加的先决条件。
The cells and tissues of patients with ataxia-telangiectasia (A-T), an inherited disease characterized by a high degree of proneness to cancer, are abnormally sensitive to ionizing radiation. Noncycling cultures of normal human and A-T fibroblasts were exposed to X-rays so that the breakage and rejoining of prematurely condensed chromosomes in the G1 phase could be compared. After a dose of 6.0 grays, both cell types had the same initial frequency of breaks and the same rate for rejoining of the breaks, but the fraction of breaks that did not rejoin was 5-6 times greater for the A-T cells. Progression of cells into the S phase is not a prerequisite for the increased frequency of chromosome fragments that appear in mitosis after A-T cells are irradiated in the G1 or G0 phase.