Hepcidin and iron species distribution inside the first-trimester human gestational sac
Hepcidin and iron species distribution inside the first-trimester human gestational sac
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DOI：
10.1093/molehr/gaq101
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发表时间：
2011-04-01
影响因子：
4
通讯作者：
Jauniaux, Eric
中科院分区：
文献类型：
--
作者：
Evans, Patricia;Cindrova-Davies, Tereza;Jauniaux, Eric
通过测定孕妇血清、体腔液和羊水中的转铁蛋白、非转铁蛋白结合铁(NTBI)和肝素原(HEP)，以及绒毛和次级卵黄囊活检组织中HEP的免疫组化染色，探讨了影响妊娠早期囊中铁分布的因素。这些样本取自妊娠8-11周的15例早期妊娠。胎儿血清转铁蛋白水平(0.56 mg/ml)显著低于母体血清水平(1.71 mg/ml)，其中CF和AF的血清转铁蛋白浓度非常低(0.09 mg/ml)。相反，胎儿的转铁蛋白饱和度(77%)显著高于母体血清(33%)。NTBI存在于胎儿血清、CF和AF中，可能是由于这些隔室中的转铁蛋白浓度较低所致。前-HEP在胎儿(140.0+/-11.1)低于母体血清(206.2+/-9.2)，在CF(19.4+/-3.1)和AF(21.8+/-5.2)中含量较低。妊娠10周时，胎盘合体滋养层、次级卵黄囊的间皮层和内胚层均可见HEP抗体免疫染色。在妊娠早期胎盘的合体滋养层细胞以及次级卵黄囊的间皮层和内胚层中，HEP的存在表明该蛋白在铁向早期胎儿的转移中起着关键的调节作用。低转铁蛋白浓度和NTBI在CF和AF中的存在表明转铁蛋白非依赖性铁转移在早期妊娠中是重要的。
We have investigated factors affecting iron distribution in the first-trimester gestational sac, by the measurement of transferrin, non-transferrin-bound iron (NTBI) and pro-hepcidin (Hep) in maternal serum, coelomic fluid (CF) and amniotic fluid (AF) and by immunostaining for Hep in villous and secondary yolk sac biopsies. These samples were obtained from 15 first-trimester pregnancies at 8-11 weeks gestation. Transferrin concentrations were significantly lower in fetal (0.56 mg/ml) than maternal serum (1.71 mg/ml), with very low concentrations in CF and AF (0.09 mg/ml). In contrast, transferrin saturations were significantly higher in fetal (77%) than maternal serum (33%). NTBI was present in fetal serum, CF and AF, presumably as a consequence of low transferrin concentrations in these compartments. Pro-Hep was present at lower levels in fetal (140.0 +/- 11.1) than maternal serum (206.2 +/- 9.2) and at low concentrations in CF (19.4 +/- 3.1) and AF (21.8 +/- 5.2). Immunostaining with Hep antibody was found in the syncytiotrophoblast of first-trimester placenta as well as in mesothelial and endodermal layers of the secondary yolk sac at 10 weeks. The presence of Hep in syncytiotrophoblast cells of first-trimester placenta as well as in mesothelial and endodermal layers of the secondary yolk sac suggest a key regulatory role for this protein in iron transfer to the first-trimester fetus. The low transferrin concentrations and the presence of NTBI in CF and AF suggest that transferrin-independent iron transfer is important in early gestation.