O2 -sensitive MRI distinguishes brain tumor versus radiation necrosis in murine models.
O2 -sensitive MRI distinguishes brain tumor versus radiation necrosis in murine models.
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DOI：
10.1002/mrm.25821
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发表时间：
2016-06
影响因子：
3.3
通讯作者：
Garbow JR
中科院分区：
文献类型：
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作者：
Beeman SC;Shui YB;Perez-Torres CJ;Engelbach JA;Ackerman JJ;Garbow JR
在正常脑、辐射诱导病变和肿瘤病变的呼吸气体激发期间，量化1H纵向弛豫速率常数R1和组织中O2浓度（弛豫率，r1）之间的关系，并量化O2驱动的R1变化（ΔR1）。弛豫度测定。在三种不同的呼吸气体（因此组织O2）条件下，在对照状态小鼠（n=4）中收集R1数据。在平行实验中，在相同的呼吸气体条件下，使用O2敏感的微探针测量对照状态小鼠（n=4）丘脑中的pO 2。使用R1和pO 2数据计算组织O2的弛豫率。病理学测定。在两种呼吸气体（因此组织O2）条件下，在对照状态（n=4）小鼠、神经胶质瘤模型（n=7）和辐射坏死模型（n=6）中收集R1数据。计算每个队列的R1和ΔR1。在4.7T时，脑内O2 r1为9×10−4±3×10−4 mmHg− 1 s −1。R1和ΔR1测量值可区分放射性坏死和肿瘤（分别为p<0.03和p<0.01）。测定了脑内O2的弛豫率。R1和ΔR1测量值可区分小鼠模型中的肿瘤病变和放射性坏死病变。这些病理很难通过传统的成像技术区分;在这方面，O2驱动的R1变化有希望。
To quantify the relationship between 1H longitudinal relaxation rate constant, R1, and O2 concentration (relaxivity, r1) in tissue and to quantify O2-driven changes in R1 (ΔR1) during a breathing gas challenge in normal brain, radiation-induced lesions, and tumor lesions. Relaxivity determination. R1 data were collected in control-state mice (n=4) during three different breathing gas (and thus tissue O2) conditions. In parallel experiments, pO2 was measured in the thalamus of control-state mice (n=4) under the same breathing gas conditions using an O2-sensitive micro-probe. The relaxivity of tissue O2 was calculated using the R1 and pO2 data. Pathology determination. R1 data were collected in control-state (n=4) mice, a glioma model (n=7), and a radiation necrosis model (n=6) during two breathing gas (thus tissue O2) conditions. R1 and ΔR1 were calculated for each cohort. O2 r1 in the brain was 9×10−4±3×10−4 mmHg−1s−1 at 4.7T. R1 and ΔR1 measurements distinguished radiation necrosis from tumor (p<0.03 and p<0.01, respectively). The relaxivity of O2 in brain is determined. R1 and ΔR1 measurements differentiate tumor lesions from radiation necrosis lesions in the mouse models. These pathologies are difficult to distinguish by traditional imaging techniques; O2-driven changes in R1 holds promise in this regard.