Microbiological profiles of sputum and gastric juice aspirates in Cystic Fibrosis patients.
Microbiological profiles of sputum and gastric juice aspirates in Cystic Fibrosis patients.
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DOI：
10.1038/srep26985
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发表时间：
2016-06-01
影响因子：
4.6
通讯作者：
Ward C
中科院分区：
文献类型：
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作者：
Al-Momani H;Perry A;Stewart CJ;Jones R;Krishnan A;Robertson AG;Bourke S;Doe S;Cummings SP;Anderson A;Forrest T;Griffin SM;Brodlie M;Pearson J;Ward C
胃食管反流（戈尔）是囊性纤维化（CF）的一个关键问题，但肺和胃微生物之间的关系还没有得到很好的理解。我们假设CF胃和肺微生物组是相关的。从15名接受经皮内镜胃造口术喂养的CF患者中获得胃和痰培养物。非CF胃液数据通过内窥镜从14例无肺部疾病的患者中获得。通过培养鉴定细菌和真菌分离株。分子细菌分析使用16 S rRNA基因的下一代测序（NGS）。培养物在所有CF胃液和胃粘膜中以及9/14的非CF胃液中生长细菌和/或真菌。11例患者CF痰中检出铜绿假单胞菌（Pa），4例患者胃中检出相同的Pa菌株。与CF样品相比，来自非CF胃液样品的NGS数据显著更多样化。NGS显示，CF胃液中的正常肠道细菌、拟杆菌和粪杆菌的丰度明显低于非CF，但假单胞菌的丰度高于非CF。多变量偏最小二乘判别分析表明，CF痰液和胃液样本的细菌谱相似，这与非CF胃液不同。我们提供了新的证据，表明CF中存在呼吸消化微生物组，这可能具有临床意义。
Gastro-Oesophageal Reflux (GOR) is a key problem in Cystic Fibrosis (CF), but the relationship between lung and gastric microbiomes is not well understood. We hypothesised that CF gastric and lung microbiomes are related. Gastric and sputum cultures were obtained from fifteen CF patients receiving percutaneous endoscopic gastrostomy feeding. Non-CF gastric juice data was obtained through endoscopy from 14 patients without lung disease. Bacterial and fungal isolates were identified by culture. Molecular bacterial profiling used next generation sequencing (NGS) of the 16S rRNA gene. Cultures grew bacteria and/or fungi in all CF gastric juice and sputa and in 9/14 non-CF gastric juices. Pseudomonas aeruginosa(Pa) was present in CF sputum in 11 patients, 4 had identical Pa strains in the stomach. NGS data from non-CF gastric juice samples were significantly more diverse compared to CF samples. NGS showed CF gastric juice had markedly lower abundance of normal gut bacteria; Bacteroides and Faecalibacterium, but increased Pseudomonas compared with non-CF. Multivariate partial least squares discriminant analysis demonstrated similar bacterial profiles of CF sputum and gastric juice samples, which were distinct from non-CF gastric juice. We provide novel evidence suggesting the existence of an aerodigestive microbiome in CF, which may have clinical relevance.