Hormones and liver mitochondria: influence of growth hormone, thyroxine, testosterone, and insulin on thermotropic effects of respiration and fatty acid composition of membranes.
Hormones and liver mitochondria: influence of growth hormone, thyroxine, testosterone, and insulin on thermotropic effects of respiration and fatty acid composition of membranes.
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激素和肝线粒体：生长激素、甲状腺素、睾酮和胰岛素对呼吸的热致效应和膜的脂肪酸组成的影响。
DOI：
10.1016/0003-9861(80)90234-9
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发表时间：
1980
影响因子：
3.9
通讯作者：
V. Maddaiah
中科院分区：
文献类型：
--
作者：
S. Clejan;P. Collipp;V. Maddaiah
在大鼠肝脏中，研究了垂体切除术和随后给予生长激素、甲状腺素、胰岛素和睾酮的影响，以研究对状态3呼吸（ADP诱导）的热致效应与完整线粒体和内膜囊泡磷脂部分脂肪酸组成之间的关系。去垂体大鼠线粒体能量链（琥珀酸盐）状态3呼吸的阿伦尼乌斯谱图与正常大鼠线粒体在23.5℃时的不连续性不同。注射激素后，在不同的温度下，脂质从凝胶状态到液晶状态的间断发生：生长激素为18.5℃，甲状腺素为26.0℃，生长激素+甲状腺素为19.5℃，胰岛素为27.6℃，睾酮为25.3℃。在37.5 - 23.5°C之间，垂体切除术的激活能量是对照组的1.9倍。生长激素在将激活能量恢复到正常状态方面是最有效的，高于和低于转变温度。甲状腺素的作用似乎是由于比生长激素、胰岛素或睾酮对状态4呼吸的刺激更大，特别是在较高的温度下。与正常大鼠相比，从完整线粒体或内膜囊泡中提取的磷脂含有较少的花生四烯酸（20:4）和较多的亚油酸（18:2）。此外，一些次要脂肪酸的含量也发生了变化。计算的不饱和指数显示，全线粒体和内膜的不饱和指数分别下降了18.8%和14.9%。在治疗垂体切除大鼠的不同激素中，生长激素在将过渡温度和脂肪酸组成恢复到正常水平和增加体重方面最有效。虽然其他激素在一定程度上增加了总不饱和指数，但对个别脂肪酸的影响不同。线粒体脂肪酸不饱和指数与状态3呼吸转变温度之间存在良好的相关性。这些结果强烈表明，激素，特别是生长激素，通过脂肪酸组成的磷脂，在垂体去切除率大鼠肝脏线粒体膜流动性的控制中的作用。
The effects of hypophysectomy and subsequent administration of growth hormone, thyroxine, insulin, and testosterone were examined in rat liver for the relationship between the thermotropic effects on State 3 respiration (ADP induced) and fatty acid composition of the phospholipid fraction of intact mitochondria as well as of inner membrane vesicles. The Arrhenius profile for energy-linked (succinate) State 3 respiration of mitochondria from hypophysectomized rats lacked the discontinuity at 23.5 °C seen with mitochondria from normal rats. After injections of the hormones the discontinuity representing the transition temperature from gel to liquid crystalline state of lipids occurred at different temperatures: 18.5 °C for growth hormone, 26.0 °C for thyroxine, 19.5 °C for growth hormone + thyroxine, 27.6 °C for insulin, and 25.3 °C for testosterone. The energy of activation between 37.5 and 23.5 °C was 1.9 times greater for hypophysectomy than for controls. Growth hormone was the most effective in restoring the energy of activation to normal, above as well as below transition temperature. The effect of thyroxine appears to be due to a larger stimulation of the State 4 respiration than that of growth hormone, insulin, or testosterone, especially at higher temperatures. Phospholipids extracted from intact mitochondria or inner membrane vesicles of hypophysectomized rats contained less arachidonic acid (20:4) and more linoleic acid (18:2) than those of normal rats. In addition, the contents of some of the minor fatty acids were also changed. Calculated unsaturation index showed an 18.8 and 14.9% depletion in unsaturation in whole mitochondria and inner membranes, respectively. Among the different hormones used to treat the hypophysectomized rats, growth hormone was the most effective in restoring the transition temperature and fatty acid composition to normal levels and increasing the gain in body weight. Although the other hormones increased total unsaturation index to some extent, some of the individual fatty acids were affected differently. Good correlation exists between the unsaturation index of mitochondrial fatty acids and transition temperature of State 3 respiration. These results strongly suggest a role for the hormones, particularly growth hormone, in the control of mitochondrial membrane fluidity of hypophysectomized rat liver, through fatty acid composition of phospholipids.