Virus induced gene silencing in Antirrhinum majus using the Cucumber mosaic virus vector: Functional analysis of the AINTEGUMENTA (Am-ANT) gene of A. majus
Virus induced gene silencing in Antirrhinum majus using the Cucumber mosaic virus vector: Functional analysis of the AINTEGUMENTA (Am-ANT) gene of A. majus
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DOI：
10.1007/s13580-011-0172-y
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发表时间：
2011-04-01
影响因子：
2.4
通讯作者：
Masuta, Chikara
中科院分区：
文献类型：
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作者：
Kim, Bo Min;Inaba, Jun-ichi;Masuta, Chikara
拟南芥基因AINTEGUMENTA（At-ANT）在细胞增殖和器官生长中起作用。ANT蛋白具有两个AP 2结构域的拷贝，R1和R2。最近，报道了金鱼草中At-ANT同源物（Am-ANT）的部分cDNA序列（Delgado-Benarroch等人，2009年）。在这里，我们使用病毒诱导的基因沉默（VIGS）来分析已报道的Am-ANT的功能。然后我们确定了Am-ANT的开放阅读框架（ORF）及其预测的氨基酸序列。我们用含有Am-ANT部分序列的黄瓜花叶病毒载体（CMV-A1）（A1：ANT）诱导VIGS，并抑制Am-ANT mRNA的水平，并观察任何表型变化。Am-ANT的功能与At-ANT非常相似。A1：ANT感染金鱼草植物有较小的花器官和叶，即使细胞大小不变的花和较大的叶。基于CMV的VIGS显示，分离的Am-ANT基因确实在细胞增殖和器官生长中起作用，如对于At-ANT所观察到的。结果表明，CMV载体具有系统感染A. majus无严重症状的功能分析。伟大的基因。
The Arabidopsis gene AINTEGUMENTA (At-ANT) functions in cell proliferation and organ growth. The ANT protein has two copies of the AP2 domains, R1 and R2. Recently, a partial cDNA sequence of the At-ANT homolog in Antirrhinum majus (Am-ANT) was reported (Delgado-Benarroch et al., 2009). Here, we used virus-induced gene silencing (VIGS) to analyze the function of the reported Am-ANT. We then determined the open reading frame (ORF) of Am-ANT and its predicted amino acid sequence. We induced VIGS using the Cucumber mosaic virus vector (CMV-A1) that contained partial sequence of Am-ANT (A1:ANT) and suppressed the level of Am-ANT mRNA and noted for any phenotypic changes. The function of Am-ANT was very similar to that of At-ANT. The A1:ANT-infected Antirrhinum plants had smaller floral organs and leaves, even though cell sizes were unchanged in flowers and larger in leaves. The CMV-based VIGS showed that the isolated Am-ANT gene was indeed functional in cell proliferation and organ growth as observed for At-ANT. In conclusion, we found that the CMV vector had the advantage of systemically infecting A. majus without severe symptoms for functional analysis of A. majus genes.