A novel PHB depolymerase from a thermophilic Streptomyces sp.
A novel PHB depolymerase from a thermophilic Streptomyces sp.
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DOI：
10.1007/s10529-005-6063-5
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发表时间：
2006-03-01
影响因子：
2.7
通讯作者：
Tokiwa, Y
中科院分区：
文献类型：
--
作者：
Calabia, BP;Tokiwa, Y
通过疏水相互作用层析和凝胶过滤纯化了嗜热链霉菌（Streptomyces sp. MG）的一种新型PHB解聚合酶。纯化酶的分子量为43 kDa， SDS-PAGE测定为41 kDa。最适pH为8.5℃，最适温度为60℃。酶在50℃和pH 6.5-8.5范围内稳定。该酶不仅能水解细菌聚酯，如聚3-羟基丁酸和聚3-羟基丁酸-co-3-羟基戊酸酯，还能水解合成脂肪族聚酯，如聚丙内酯、聚己二酸乙烯和聚丁二酸乙烯。
A novel PHB depolymerase from a thermophilic Streptomyces sp. MG was purified to homogeneity by hydrophobic interaction chromatography and gel filtration. The molecular mass of the purified enzyme was 43 kDa as determined by size exclusion chromatography and 41 kDa by SDS-PAGE. The optimum pH and temperature were 8.5 and 60 degrees C respectively. The enzyme was stable at 50 degrees C and from pH 6.5-8.5. The enzyme hydrolyzed not only bacterial polyesters, i.e. poly(3-hydroxybutyric acid and poly(3-hydroxybutyrate-co-3-hydroxyvalerate), but also synthetic, aliphatic polyesters such as polypropiolactone, poly(ethylene adipate) and poly(ethylene succinate).