THE EFFECTS OF MUSCLE-CONTRACTION AND INSULIN ON GLUCOSE-TRANSPORTER TRANSLOCATION IN RAT SKELETAL-MUSCLE
THE EFFECTS OF MUSCLE-CONTRACTION AND INSULIN ON GLUCOSE-TRANSPORTER TRANSLOCATION IN RAT SKELETAL-MUSCLE
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DOI：
10.1042/bj2970539
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发表时间：
1994-02-01
影响因子：
4.1
通讯作者：
IVY, JL
中科院分区：
文献类型：
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作者：
BROZINICK, JT;ETGEN, GJ;IVY, JL
在雌性SD大鼠后肢灌流过程中，研究了电诱导肌肉收缩、胰岛素(10M单位/ml)和在有胰岛素存在的情况下电诱导肌肉收缩对胰岛素可调节葡萄糖转运体(GLUT-4)蛋白分布的影响。当收缩、胰岛素或胰岛素+收缩时，质膜细胞松弛素B结合量增加2倍，而GLUT-4蛋白浓度增加1.5倍。微体-膜细胞松弛素B结合量和GLUT-4蛋白浓度降低了约1/4。30%的胰岛素或胰岛素+收缩，但不明显减少单独收缩。通过测定比目鱼肌、足底肌及腓肠肌红色和白色部分的2-脱氧[H-3]葡萄糖蓄积率来评估肌肉葡萄糖摄取率。收缩和胰岛素都显著增加了肌肉中葡萄糖的摄取，而且增加的程度相同。胰岛素+收缩使葡萄糖摄取量高于胰岛素或单独收缩，但这种影响仅在比目鱼肌、足底肌和白色腓肠肌中有统计学意义。在所研究的肌肉中，胰岛素+收缩葡萄糖摄取的联合效应并不是完全相加的。这些结果表明：(1)胰岛素和肌肉收缩正在动员两个不同的GLUT-4蛋白池，(2)由于胰岛素+收缩导致的骨骼肌葡萄糖摄取增加并不是由于质膜GLUT-4蛋白浓度高于胰岛素或单独收缩的观察结果。
The effect of electrically induced muscle contraction, insulin (10 m-units/ml) and electrically-induced muscle contraction in the presence of insulin on insulin-regulatable glucose-transporter (GLUT-4) protein distribution was studied in female Sprague-Dawley rats during hindlimb perfusion. Plasma-membrane cytochalasin B binding increased similar to 2-fold, whereas GLUT-4 protein concentration increased similar to 1.5-fold above control with contractions, insulin, or insulin + contraction. Microsomal-membrane cytochalasin B binding and GLUT-4 protein concentration decreased by approx. 30% with insulin or insulin + contraction, but did not significantly decrease with contraction alone. The rate of muscle glucose uptake was assessed by determining the rate of 2-deoxy[H-3]glucose accumulation in the soleus, plantaris, and red and white portions of the gastrocnemius. Both contraction and insulin increased glucose uptake significantly and to the same degree in the muscles examined. Insulin + contraction increased glucose uptake above that of insulin or contraction alone, but this effect was only statistically significant in the soleus, plantaris and white gastrocnemius. The combined effects of insulin + contraction of glucose uptake were not fully additive in any of the muscles investigated. These results suggest that (1) insulin and muscle contraction are mobilizing two separate pools of GLUT-4 protein, and (2) the increase in skeletal-muscle glucose uptake due to insulin + contraction is not due to an increase in plasma-membrane GLUT-4 protein concentration above that observed for insulin or contraction alone.