POPULATION-DEPENDENT REQUIREMENT BY CULTURED MAMMALIAN CELLS FOR METABOLITES WHICH THEY CAN SYNTHESIZE
POPULATION-DEPENDENT REQUIREMENT BY CULTURED MAMMALIAN CELLS FOR METABOLITES WHICH THEY CAN SYNTHESIZE
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DOI：
10.1084/jem.116.1.29
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发表时间：
1962-01-01
影响因子：
15.3
通讯作者：
PIEZ, K
中科院分区：
文献类型：
--
作者：
EAGLE, H;PIEZ, K
培养的细胞合成的化合物的数量应足以维持细胞的持续生长，这已被证明是在某些条件下生存所必需的（天冬酰胺、胱氨酸、谷氨酰胺、高胱氨酸、肌醇、丙酮酸和丝氨酸），这种需要取决于种群数量。当细胞密度足够大，使培养基和细胞池中的浓度达到代谢有效水平时，它就会消失，然后细胞就会因特定缺陷而死亡。允许细胞存活和生长的临界群体密度超过200，000个细胞/ml。
Compounds synthesized by cultured cells in amounts which should suffice for sustained growth have proved necessary under certain conditions for survival (asparagine, cystine, glutamine, homocystine inositol, pyruvate and serine).That requirement is population-dependent. It disappears at cell densities sufficiently large to bring the concentration in the medium and in the cellular pool to metabolically effective levels before the cells died of the specific deficiency. The critical population density permitting cellular survival and growth was in excess of 200,000 cells/ml.