Resistance mechanisms and genetic relatedness among carbapenem-resistant Pseudomonas aeruginosa isolates from three major hospitals in Hanoi, Vietnam (2011-15).
Resistance mechanisms and genetic relatedness among carbapenem-resistant Pseudomonas aeruginosa isolates from three major hospitals in Hanoi, Vietnam (2011-15).
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DOI：
10.1093/jacamr/dlab103
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发表时间：
2021-09
影响因子：
3.4
通讯作者：
van Doorn HR
中科院分区：
文献类型：
--
作者：
Tran HA;Vu TNB;Trinh ST;Tran DL;Pham HM;Ngo THH;Nguyen MT;Tran ND;Pham DT;Dang DA;Shibayama K;Suzuki M;Yoshida LM;Trinh HS;Le VT;Vu PT;Luu TVN;Bañuls AL;Trinh KL;Tran VA;Tran HH;van Doorn HR
MDR细菌包括耐碳青霉烯类铜绿假单胞菌被认为是全球医院获得性感染的重要原因。本研究旨在确定与碳青霉烯类耐药铜绿假单胞菌相关的分子特征和抗生素耐药基因。我们对2011年8月至2015年3月在越南河内三家主要医院从医院获得性感染患者中分离的72株碳青霉烯类耐药铜绿假单胞菌进行了WGS和系统发育分析。我们鉴定了IMP基因的三种变体，其中blaIMP-15是最常见的（n = 34），而blaIMP-26（n = 2）和blaIMP-51（n = 12）。我们观察到两株亚胺培南MIC >128 mg/L的分离株同时携带blaIMP-15和blaDIM-1基因，7株亚胺培南MIC > 128 mg/L的分离株携带blaKPC-1基因。MLST数据显示，这72个菌株属于18个ST，系统发育树分析将这些菌株分为9个组。我们的研究结果提供的证据表明，不仅blaIMP-26，但其他IMP变异，如blaIMP-15和blaIMP-51基因和几个ST（ST 235，ST 244，ST 277，ST 310，ST 773和ST 3151）已在越南的医疗机构传播。此外，我们报告了在越南出现的两种属于ST 1240和ST 3340的分离株（携带两种重要的碳青霉烯酶基因（blaIMP-15和blaDIM-1））和七种属于ST 3151的铜绿假单胞菌分离株（携带blaKPC-1基因），这可能导致医疗机构治疗选择的可用性严重受限。
MDR bacteria including carbapenem-resistant Pseudomonas aeruginosa are recognized as an important cause of hospital-acquired infections worldwide. This investigation seeks to determine the molecular characterization and antibiotic resistance genes associated with carbapenem-resistant P. aeruginosa. We conducted WGS and phylogenetic analysis of 72 carbapenem-resistant P. aeruginosa isolated from hospital-acquired infection patients from August 2011 to March 2015 in three major hospitals in Hanoi, Vietnam. We identified three variants of IMP gene, among which blaIMP-15 was the most frequent (n = 34) in comparison to blaIMP-26 (n = 2) and blaIMP-51 (n = 12). We observed two isolates with imipenem MIC >128 mg/L that co-harboured blaIMP-15 and blaDIM-1 genes and seven isolates (imipenem MIC > 128 mg/L) with a blaKPC-1 gene from the same hospital. MLST data shows that these 72 isolates belong to 18 STs and phylogenetic tree analysis has divided these isolates into nine groups. Our results provide evidence that not only blaIMP-26 but other IMP variants such as blaIMP-15 and blaIMP-51 genes and several STs (ST235, ST244, ST277, ST310, ST773 and ST3151) have been disseminating in healthcare settings in Vietnam. In addition, we report the emergence of two isolates belonging to ST1240 and ST3340 that harboured two important carbapenemase genes (blaIMP-15 and blaDIM-1) and seven isolates belonging to ST3151 of P. aeruginosa that carried the blaKPC-1 gene in Vietnam, which could potentially cause serious restricted availability of treatment options in healthcare settings.