An amyloid-β targeting chemical exchange saturation transfer probe for in vivo detection of Alzheimer's disease.
An amyloid-β targeting chemical exchange saturation transfer probe for in vivo detection of Alzheimer's disease.
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DOI：
10.1021/acschemneuro.9b00334
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发表时间：
2019-07
影响因子：
5
通讯作者：
Runrun Wang;Chen-Wei Wang;Z. Dai;Yanzi Chen;Zhiwei Shen;G. Xiao;Yuanfeng Chen;Jiang-Ning Zhou
中科院分区：
文献类型：
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作者：
Runrun Wang;Chen-Wei Wang;Z. Dai;Yanzi Chen;Zhiwei Shen;G. Xiao;Yuanfeng Chen;Jiang-Ning Zhou
可靠、重复性好的Aβ沉积物检测有助于阿尔茨海默病（AD）的早期诊断。本研究以angiopep-2为探针，探讨了化学交换饱和转移法（CEST）检测Aβ存款的可行性，并证明CEST在体外可检测到angiopep-2和Aβ-angiopep-2聚集体。此外，与对照相比，注射血管肽素-2的APP/PS1小鼠表现出显著更高的体内CEST效应。CEST成像检测到的Aβ沉积物分布与组织学染色结果一致。本研究首次报道了一种可靠的外源性CEST探针，用于非侵入性评估APP/PS1小鼠中的Aβ沉积。此外，这些结果证明了使用Angiopep-2探针进行CEST成像进行临床AD诊断和Aβ靶向药物治疗评估的潜力。
A reliable and reproducible detection of Aβ deposits would be beneficial for the early diagnosis of Alzheimer's disease (AD). In the present study, the feasibility of applying chemical exchange saturation transfer (CEST) for Aβ deposit detection using angiopep-2 as a probe was evaluated, and it was demonstrated that CEST could detect angiopep-2 and Aβ-angiopep-2 aggregates in vitro. Furthermore, APP/PS1 mice injected with angiopep-2 exhibited a significantly higher in vivo CEST effect, when compared to controls. The distribution of Aβ deposits detected by CEST imaging was consistent with the histological staining results. The present study is the first to report a reliable exogenous CEST probe to noninvasively evaluate Aβ deposits in APP/PS1 mice. Furthermore, these results demonstrate the potential for clinical AD diagnosis and Aβ-targeted drug therapy assessment using CEST imaging with the angiopep-2 probe.