Dynactin increases the processivity of the cytoplasmic dynein motor
Dynactin increases the processivity of the cytoplasmic dynein motor
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DOI：
10.1038/71338
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发表时间：
2000-01-01
影响因子：
21.3
通讯作者：
Schroer, TA
中科院分区：
文献类型：
--
作者：
King, SJ;Schroer, TA
细胞质动力蛋白支持货物在体内的长距离细胞内运动，但本身似乎不是一种进行性运动蛋白。我们在这里表明，动力蛋白激活剂，dynactin，结合微管，并增加细胞质动力蛋白驱动的运动的平均长度，而不影响速度或微管刺激的ATP酶动力学的细胞质动力蛋白。由dynactin引起的微管结合和运动的增强都被dynactin微管结合域的抗体抑制。这些结果表明，dynactin作为一个持续性因素的细胞质动力蛋白为基础的运动，并提供了第一个证据表明，细胞骨架运动持续性可以受到外在因素的影响。
Cytoplasmic dynein supports long-range intracellular movements of cargo in vivo but does not appear to be a processive motor protein by itself. We show here that the dynein activator, dynactin, binds microtubules and increases the average length of cytoplasmic-dynein-driven movements without affecting the velocity or microtubule-stimulated ATPase kinetics of cytoplasmic dynein. Enhancement of microtubule binding and motility by dynactin are both inhibited by an antibody to dynactin's microtubule-binding domain. These results indicate that dynactin acts as a processivity factor for cytoplasmic-dynein-based motility and provide the first evidence that cytoskeletal motor processivity can be affected by extrinsic factors.