Characterization of a thyroid hormone receptor expressed in human kidney and other tissues.
Characterization of a thyroid hormone receptor expressed in human kidney and other tissues.
复制标题
人类肾脏和其他组织中表达的甲状腺激素受体的表征。
DOI：
10.1073/pnas.85.8.2781
复制
发表时间：
1988
影响因子：
11.1
通讯作者：
DeGroot,LJ
中科院分区：
文献类型：
--
作者：
Nakai,A;Seino,S;Sakurai,A;Szilak,I;Bell,GI;DeGroot,LJ
从人肾文库中分离到一种编码人肝、肾、胎盘和脑中表达的特定形式甲状腺激素受体的cDNA。在人胎盘和HepG2 cDNA文库中发现了相同的克隆。该cDNA编码490个氨基酸的蛋白（Mr, 54,824）。在体外表达和翻译时，该蛋白产物与三碘甲状腺原氨酸结合，Ka为2.3 X 10(9) M-1。该蛋白被命名为人类甲状腺激素受体α 2型（hTR α 2），与v- erba相关受体基因超家族的其他成员具有相同的结构域结构。它与鸡和大鼠的α型甲状腺激素受体相似，而与胎盘中的人类β型甲状腺激素受体（以前称为c-erbA β）不太相似。然而，它与这些受体的区别在于其C端激素结合域的延伸，使其比大鼠甲状腺激素受体α 1型长80个氨基酸[Thompson， c.c., Weinberger， C, Lebo， R. & Evans， R. M. (1987) Science 237, 1610-1614]。在肝脏（2.5千碱基）和肾脏（2千碱基）中发现的mRNA大小不同，表明该基因的初级转录本可能存在组织特异性加工。人类甲状腺激素受体α 2型的鉴定表明，在人体组织中存在两种或两种以上形式的甲状腺激素受体，并可能解释甲状腺激素抵抗综合征中各器官甲状腺激素反应性的正常变化和选择性组织异常。
A cDNA encoding a specific form of thyroid hormone receptor expressed in human liver, kidney, placenta, and brain was isolated from a human kidney library. Identical clones were found in human placenta and HepG2 cDNA libraries. The cDNA encodes a 490-amino acid protein (Mr, 54,824). When expressed and translated in vitro, the protein product binds triiodothyronine with Ka of 2.3 X 10(9) M-1. This protein, designated human thyroid hormone receptor type alpha 2 (hTR alpha 2), has the same domain structure as other members of the v-erbA-related superfamily of receptor genes. It is similar to thyroid hormone receptor type alpha described in chicken and rat and less similar to human thyroid hormone receptor type beta (formerly referred to as c-erbA beta) from placenta. However, it is distinguished from these receptors by an extension of the C-terminal hormone binding domain making it 80 amino acids longer than rat thyroid hormone receptor type alpha 1 [Thompson, C.C., Weinberger, C., Lebo, R. & Evans, R. M. (1987) Science 237, 1610-1614]. Different sizes of mRNA found in liver (2.5 kilobases) and kidney (2 kilobases) suggest there may be tissue-specific processing of the primary transcript of this gene. Identification of human thyroid hormone receptor type alpha 2 indicates that two or more forms of thyroid hormone receptor exist in human tissues and may explain the normal variation in thyroid hormone responsiveness of various organs and the selective tissue abnormalities found in the thyroid hormone resistance syndromes.