Virus inactivation of plasma-derived proteins by pasteurization in the presence of guanidine hydrochloride
Virus inactivation of plasma-derived proteins by pasteurization in the presence of guanidine hydrochloride
复制标题
DOI：
10.1046/j.1537-2995.2001.41030382.x
复制
发表时间：
2001-03-01
期刊：
影响因子：
2.9
通讯作者：
Kempf, C
中科院分区：
文献类型：
--
作者：
Schlegel, A;Immelmann, A;Kempf, C
背景技术背景：病毒，其中包括细小病毒B19和其他小的无包膜病毒，可能存在于人血液中，并可能污染血浆来源的治疗剂。有效灭活或去除此类病毒，尤其是细小病毒，是当前的一个问题，相应的技术正在研究中。在这份报告中，这样的技术是described.Study设计和方法：最近开发的巴氏杀菌的人载脂蛋白A-I（apoA-I），这是在60 degreesC进行10小时的盐酸胍（GdnHCl）的存在下，通过使用一系列的模型病毒，包括家庭细小病毒科和小核糖核酸病毒科的成员进行了验证。将模型病毒加标至含apoA-I和GdnHCl的溶液中，并通过细胞培养物中的感染性试验评价病毒灭活。病毒灭活的机制进行了研究，通过病毒沉降分析使用picornavirusmodel.RESULTS：所有的病毒测试被灭活到检测限以下的水平，虽然不同的病毒得到不同的灭活动力学。这种巴氏灭菌法灭活病毒的机制是将病毒颗粒拆解成单个蛋白质或小的非感染性病毒subunit.CONCLUSION：本报告中验证的巴氏灭菌法有可能灭活广泛的输血相关病毒，包括细小病毒和小核糖核酸病毒。
BACKGROUND: Viruses, among them parvovirus B19 and other small, nonenveloped viruses, may be present in human blood and may contaminate plasma-derived therapeutics. Efficient inactivation or removal of such viruses, especially parvoviruses, represents a current problem and corresponding technologies are under investigation. In this report, such a technology is described.STUDY DESIGN AND METHODS: A recently developed pasteurization of human apolipoprotein A-I (apoA-I), which is performed at 60 degreesC for 10 hours in the presence of guanidine hydrochloride (GdnHCl), was validated by using a series of model viruses, including members of the families parvoviridae and picornaviridae. The model viruses were spiked into the apoA-I- and GdnHCl-containing solutions, and virus inactivation was evaluated by infectivity assays in cell cultures. The mechanism of virus inactivation was studied by virus sedimentation analysis using the picornavirus model.RESULTS: All viruses tested were inactivated to levels below the limit of detection, although different inactivation kinetics were obtained for the different viruses. The mechanism of virus inactivation by this pasteurization was disassembly of the virus particles into single proteins or small noninfectious viral subunits.CONCLUSION: The pasteurization validated in this report has the potential to inactivate a wide range of transfusion-relevant viruses including parvoviruses and picornaviruses.