Attenuation of obesity-induced inflammation in mice orally administered with salmon cartilage proteoglycan, a prophylactic agent
Attenuation of obesity-induced inflammation in mice orally administered with salmon cartilage proteoglycan, a prophylactic agent
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DOI：
10.1016/j.bbrc.2017.01.056
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发表时间：
2017-03-11
影响因子：
3.1
通讯作者：
Nakane, Akio
中科院分区：
文献类型：
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作者：
Hirose, Shouhei;Asano, Krisana;Nakane, Akio
肥胖与脂肪组织的慢性炎症有关，并导致2型糖尿病的发展。肥胖小鼠脂肪组织中M1巨噬细胞数量增加。M1巨噬细胞通过分泌促炎细胞因子诱导胰岛素抵抗。我们之前的研究表明，鲑鱼软骨蛋白聚糖（PG）可以抑制各种小鼠炎症性疾病中的过度炎症。在这项研究中，我们研究了PG对2型糖尿病的影响，采用高脂饮食（HFD）诱导的肥胖小鼠模型。口服PG给药可增加小脂肪细胞（面积小于1000 μ m（2））的数量，而不会增加体重和组织重量。此外，PG给药抑制了脂肪组织中TNF-α、IL-6和CXCL 2的mRNA表达。PG给药可降低M1巨噬细胞的比例。此外，PG给药抑制腹膜内葡萄糖注射后的高血糖症。PG给药小鼠的空腹血清胰岛素水平降低。此外，胰岛素刺激的Akt磷酸化增强的PG给药小鼠的肝脏和腓肠肌骨骼肌。这些数据表明，PG管理改善高血糖症和胰岛素敏感性肥胖小鼠的M1巨噬细胞分泌促炎细胞因子在脂肪组织和激活Akt在肝脏和骨骼肌的调制。(C)2017爱思唯尔公司All rights reserved.
Obesity is associated with chronic inflammation of adipose tissue and causes development of type 2 diabetes. M1 macrophage population was increased in adipose tissue of obese mouse. M1 macrophages induce insulin resistance through the secretion of proinflammatory cytokines. Our previous studies demonstrated that salmon cartilage proteoglycan (PG) suppresses excess inflammation in various mouse inflammatory diseases. In this study, we examined the effect of PG on type 2 diabetes using high-fat-diet (HFD) induced obese mouse model. Oral PG administration enhanced the population of small adipocytes (area less than 1000 mu m(2)) without body and tissue weight gain. In addition, PG administration suppressed mRNA expression of TNF-alpha, IL-6 and CXCL2 in adipose tissue. The proportion of M1 macrophages was decreased by PG administration. In addition, PG administration suppressed hyperglycemia after intraperitoneal glucose injection. Fasted serum insulin level was decreased in PG-administered mice. Moreover, insulin-stimulated phosphorylation of Akt was enhanced in the liver and gastrocnemius skeletal muscle of PG-administered mice. These data suggested that PG administration improves hyperglycemia and insulin sensitivity in obese mice by modulation of M1 macrophages which secrete proinflammatory cytokines in adipose tissue and activation of Akt in liver and skeletal muscle. (C) 2017 Elsevier Inc. All rights reserved.