A novel near-infrared fluorescent probe for H2O2 in alkaline environment and the application for H2O2 imaging in vitro and in vivo
A novel near-infrared fluorescent probe for H2O2 in alkaline environment and the application for H2O2 imaging in vitro and in vivo
复制标题
一种新型碱性环境下H2O2近红外荧光探针及其在体外和体内H2O2成像中的应用
DOI：
10.1016/j.biomaterials.2016.05.029
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发表时间：
2016-09-01
期刊：
影响因子：
14
通讯作者：
Lin, Weiying
中科院分区：
文献类型：
--
作者：
Liu, Keyin;Shang, Huiming;Lin, Weiying
H_2O_2作为最重要的活性氧物种之一，在碱性环境中对DNA、RNA、蛋白质和酶等生物分子具有更强的攻击活性，从而导致一系列疾病的发生。然而，以往对碱性环境中H_2O_2的荧光检测还没有引起足够的重视。在此，我们报道了第一个基于长沙近红外染料(CSBOH)硼酸衍生物的比率近红外荧光探针在碱性条件下检测H_2O_2及其在活体H_2O_2成像中的应用。在CSBOH中利用ICT(分子内电荷转移)机制来调节荧光变化。通过与含酚结构类似物长沙近红外染料(CSOH)的苯酚衍生物的比较，研究了CSBOH的光物理变化。在H_2O_2存在下，CSBOH在碱性缓冲溶液中分别被560 nm和670 nm光激发时，在650 nm和720 nm处的荧光变化明显不同，对H_2O_2具有较高的选择性。细胞实验表明，CSBOH可以成像巨噬细胞和A431细胞中内源性产生的过氧化氢。体内实验表明，CSOH和CSBOH都可以用于生物成像，CSBOH可以成功地对活体动物体内的过氧化氢进行成像。(C)2016爱思唯尔有限公司。保留所有权利。
H2O2 as one of the most important ROS (Reactive Oxygen Species) has more attack activity to bio-molecules such as DNA, RNA, protein and enzyme in alkaline environment and leads to a series of disease. However, no attention has been paid to the fluorescent detection of H2O2 in alkaline environment in the past. Herein, we reported the first ratiometric near-infrared fluorescent probe based on a boric acid derivative of Changsha near-infrared dye (CSBOH) for H2O2 detection in alkaline condition and the application for H2O2 imaging in vivo. ICT (intra-molecular charge transfer) mechanism was used in CSBOH to modulate the fluorescence change. The photophysical change of CSBOH was investigated by comparison with a phenol derivative of Changsha near-infrared dye (CSOH), a structural analogue bearing phenol group. In the presence of H2O2, CSBOH exhibited remarkably different fluorescence change at 650 nm and 720 nm when excited by 560 nm and 670 nm light respectively in alkaline buffer and showed high selectivity toward H2O2. Cellular experiments demonstrate that CSBOH can image endogenously generated H2O2 in macrophages and A431 cells. In vivo experiment demonstrates that both CSOH and CSBOH can be used for bio-imaging, and CSBOH can image H2O2 in living animal successfully. (C) 2016 Elsevier Ltd. All rights reserved.