The largemouth bass cytochrome b gene
The largemouth bass cytochrome b gene
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DOI：
10.1111/j.1095-8649.1994.tb01240.x
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发表时间：
1994-04
影响因子：
2
通讯作者：
D. Whitmore;T. Thai;C. Craft
中科院分区：
文献类型：
--
作者：
D. Whitmore;T. Thai;C. Craft
细胞色素B基因是线粒体基因组的蛋白质编码基因之一，由于分子技术可以容易地应用于其结构的分析而变得重要。大嘴鲈鱼（Microplerus salmoides）细胞色素B基因的核苷酸序列的测定和推断的氨基酸结构的形式最初来自大鼠细胞色素B的结构模型。在该模型的背景下，对来自不同动物物种的推断氨基酸序列进行比对和比较。这些数据表明，由于与功能限制相关的不同选择压力，基因的区域可能以不同的速度进化。细胞色素B的保守区和可变区已被确定，并可以在未来的研究中用于物种鉴定，种内变异的检查和系统发育重建。
The cytochrome b gene is one of the protein-coding genes of the mitochondrial genome that has gained importance because of the ease with which molecular techniques can be applied to the analysis of its structure. The nucleotide sequence of the largemouth bass (Microplerus salmoides) cytochrome b gene was determined and the inferred amino acid structure is presented in the form of a structural model derived originally from rat cytochrome b. The inferred amino acid sequences from divergent animal species are aligned and compared in the context of this model. The data suggest that regions of the gene may be evolving at different rates due to different selection pressures associated with functional constraints. Conserved and variable regions of cytochrome b have been identified and can be targeted for species identification, the examination of intraspeciflc variation, and phylogenetic reconstructions in future research.