Dietary ginger constituents, galanals A and B, are potent apoptosis inducers in Human T lymphoma Jurkat cells
Dietary ginger constituents, galanals A and B, are potent apoptosis inducers in Human T lymphoma Jurkat cells
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DOI：
10.1016/s0304-3835(03)00381-1
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发表时间：
2003-09-25
期刊：
影响因子：
9.7
通讯作者：
Osawa, T
中科院分区：
文献类型：
--
作者：
Miyoshi, N;Nakamura, Y;Osawa, T
本文研究了姜类化合物姜黄素、6-姜酚和劳丹烷型二萜类化合物对培养的人T淋巴瘤Jurkat细胞增殖和诱导凋亡的作用。在测试的化合物中，Galanals A和13，分离自日本姜，myoga（花柏mioga Roscoe）的花蕾，显示出最有效的细胞毒性作用。Jurkat人T细胞白血病细胞暴露于甘丙肽导致诱导以DNA片段化和半胱天冬酶-3激活为特征的凋亡性细胞死亡。线粒体损伤途径被认为参与了甘丙肽诱导的细胞凋亡，因为用甘丙肽处理细胞诱导线粒体跨膜电位（Deltapsim）改变和细胞色素c释放。Galanal处理后，Bcl-2蛋白表达下调，Bax蛋白表达增强。总之，这项研究的结果提供了生物学证据，表明姜黄素以外的生姜特异性成分是潜在的抗癌剂。(C)2003爱思唯尔爱尔兰有限公司保留所有权利。
The effects of the constituents isolated from ginger species including curcumin, 6-gingerol and labdane-type diterpene compounds on cell proliferation and the induction of apoptosis in the cultured human T lymphoma Jurkat cells were studied. Among the tested compounds, Galanals A and 13, isolated from the flower buds of a Japanese ginger, myoga (Zingiber mioga Roscoe), showed the most potent cytotoxic effect. Exposure Of Jurkat human T-cell leukemia cells to galanals resulted in the induction of apoptotic cell death characterized by DNA fragmentation and caspase-3 activation. The mitochondrial damage pathway was suggested to be involved in galanal-induced apoptosis because the treatment of cells with galanals induced mitochondrial transmembrane potential (Deltapsim) alteration and cytochrome c release. The anti-apoptotic Bcl-2 protein was downregulated by the galanal treatment together with enhancement of the Bax expression. In conclusion, the results from this study provide biological evidence that ginger-specific constituents other than curcuminoids are potential anticancer agents. (C) 2003 Elsevier Ireland Ltd. All rights reserved.